The adipose tissue of origin influences the biological potential of human adipose stromal cells isolated from mediastinal and subcutaneous fat depots

Camilla Siciliano, Antonella Bordin, Mohsen Ibrahim, Isotta Chimenti, Francesco Cassiano, Ilenia Gatto, Giorgio Mangino, Andrea Coccia, Selenia Miglietta, Daniela Bastianelli, Vincenzo Petrozza, Antonella Calogero, Giacomo Frati, Elena De Falco

Research output: Contribution to journalArticle

Abstract

Indirect evidence suggests that adipose tissue-derived stromal cells (ASCs) possess different physiological and biological variations related to the anatomical localization of the adipose depots. Accordingly, to investigate the influence of the tissue origin on the intrinsic properties of ASCs and to assess their response to specific stimuli, we compared the biological, functional and ultrastructural properties of two ASC pools derived from mediastinal and subcutaneous depots (thoracic compartment) by means of supplements such as platelet lysate (PL) and FBS. Subcutaneous ASCs exhibited higher proliferative and clonogenic abilities than mediastinal counterpart, as well as increased secreted levels of IL-6 combined with lower amount of VEGF-C. In contrast, mediastinal ASCs displayed enhanced pro-angiogenic and adipogenic differentiation properties, increased cell diameter and early autophagic processes, highlighted by electron microscopy. Our results further support the hypothesis that the origin of adipose tissue significantly defines the biological properties of ASCs, and that a homogeneric function for all ASCs cannot be assumed.

Original languageEnglish
Pages (from-to)342-351
Number of pages10
JournalStem Cell Research
Volume17
Issue number2
DOIs
Publication statusPublished - Sep 1 2016

Keywords

  • Adipose tissue
  • Mesenchymal stem cells
  • Platelet lysate angiogenesis
  • Transmission electron microscopy

ASJC Scopus subject areas

  • Medicine(all)
  • Developmental Biology
  • Cell Biology

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    Siciliano, C., Bordin, A., Ibrahim, M., Chimenti, I., Cassiano, F., Gatto, I., Mangino, G., Coccia, A., Miglietta, S., Bastianelli, D., Petrozza, V., Calogero, A., Frati, G., & De Falco, E. (2016). The adipose tissue of origin influences the biological potential of human adipose stromal cells isolated from mediastinal and subcutaneous fat depots. Stem Cell Research, 17(2), 342-351. https://doi.org/10.1016/j.scr.2016.07.010