The AGMA1 polyamidoamine mediates the efficient delivery of siRNA

Roberta Cavalli, Luca Primo, Roberto Sessa, Giulia Chiaverina, Laura di Blasio, Jenny Alongi, Amedea Manfredi, Elisabetta Ranucci, Paolo Ferruti

Research output: Contribution to journalArticle

Abstract

AGMA1, a prevailingly cationic, guanidine-bearing, linear, amphoteric polyamidoamine is an effective siRNA condensing agent. Here two AGMA1 samples of different molecular weight, i.e. AGMA1–5 and AGMA1–10 were evaluated as siRNA condensing agents and transfection promoters. AGMA1–10 formed stable polyplexes with a size lower than 50 nm and positive zeta potential. AGMA1–5 polyplexes were larger, about 100 nm in size. AGMA1–10 polyplexes, but not AGMA1–5 proved to be an effective intracellular siRNA carrier, able to trigger gene silencing in Hela and PC3 cell lines without eliciting cytotoxic effects. AGMA1–10 knocked down AKT-1 expression upon transfection with an AKT-1 specific siRNA. The polyplex entry mechanism was investigated and was mediated by macropinocytosis. In conclusion, AGMA1 has potential as an efficient, non-toxic tool for the intracellular delivery of siRNA and warrants further investigation.

Original languageEnglish
Pages (from-to)891-898
Number of pages8
JournalJournal of Drug Targeting
Volume25
Issue number9-10
DOIs
Publication statusPublished - Nov 26 2017

Keywords

  • AGMA1
  • AGMA1/siRNA polyplexes
  • gene silencing
  • intracellular siRNA delivery
  • Polyamidoamine
  • siRNA

ASJC Scopus subject areas

  • Pharmaceutical Science

Fingerprint Dive into the research topics of 'The AGMA1 polyamidoamine mediates the efficient delivery of siRNA'. Together they form a unique fingerprint.

Cite this