The antimicrobial peptide Tachyplesin III coated alone and in combination with intraperitoneal piperacillin-tazobactam prevents ureteral stent Pseudomonas infection in a rat subcutaneous pouch model

Daniele Minardi, Roberto Ghiselli, Oscar Cirioni, Andrea Giacometti, Wojciech Kamysz, Fiorenza Orlando, Carmela Silvestri, Gianni Parri, Elzbieta Kamysz, Giorgio Scalise, Vittorio Saba, Muzzonigro Giovanni

Research output: Contribution to journalArticlepeer-review

Abstract

We investigated the efficacy of Tachyplesin III alone or combined with piperacillin-tazobactam (TZP) to prevent biofilm formation in vitro and in a rat model of Pseudomonas aeruginosa ureteral stent infection. We have observed that in vitro TZP, in presence of Tachyplesin III, showed minimal inhibitory concentrations (MIC)s twofold and minimal bactericidal concentrations (MBC)s eightfold lower. The in vivo study showed that rats that received intraperitoneal TZP showed the lowest bacterial numbers. Tachyplesin III combined with TZP showed efficacies higher than that of each single compound. Coating ureteral stents with Tachyplesin III is able to inhibit bacterial growth up to 1000 times.

Original languageEnglish
Pages (from-to)2293-2298
Number of pages6
JournalPeptides
Volume28
Issue number12
DOIs
Publication statusPublished - Dec 2007

Keywords

  • Bacterial adhesion
  • Biofilm
  • Peptides
  • Tachyplesin III
  • Ureteral stents
  • Urinary tract infections

ASJC Scopus subject areas

  • Biochemistry
  • Endocrinology
  • Physiology
  • Cellular and Molecular Neuroscience

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