Objective: To test the antioxidant effect of therapeutic doses of dipyridamole on cellular membranes, human erythrocytes were chosen as an appropriate model to study oxidative stress induced by cumene hydroperoxide because of their high content in heme-Fe 2+. Methods: The oxidative stress was induced by incubation with 160 μmoll -1 cumene hydroperoxide and expressed by three main factors: lipid peroxidation by means of kinetics of decrease in fluorescence emission of the probe incorporated in the cell membranes, vitamin E oxidation and intracellular thiol content. The concentrations of dipyridamole tested (2-20 μmoll -1) did not exceed pharmacological doses. Results. After 7 min of incubation at 25°C with the oxidant and 20 μmoll -1 dipyridamole thiol content was 50.1% ± 2.6 compared with 31.5% ± 2.4 in the absence of the drug. After 12 min vitamin E content was 88.3% ± 2.3 compared with 64.7% ± 3.4 of untreated cells in the absence of dipyridamole. Dipyridamole added 5 min after the oxidation reaction suppressed the fluorescence decrease for a time proportional to the drug concentration. Conclusions. Thus, at clinically realistic doses dipyridamole shows a concentration-dependent antioxidant effect. It protects membranes from oxidation and spares the antioxidant power of erythrocytes. (C) 2000 Elsevier Science B.V.
- Free radicals
ASJC Scopus subject areas
- Cardiology and Cardiovascular Medicine