Abstract
Ischemia is associated with myocardial acidosis which recovers upon reperfusion. In such conditions, alpha 1-adrenergic stimulation is arrhythmogenic. We used single cardiac myocytes loaded with the pH fluorescent dye, SNARF-1, to determine if a modulation of pH could explain the effect of alpha 1-adrenergic stimulation. Cells were exposed to acidosis (CO2 15%) for 15 min and then normocapnia restored. During acidosis, alpha 1-adrenergic stimulation caused an increase in pH which was abolished by blocking Na+/H+ exchange with ethylisopropylamiloride (EIPA). After removal of acidosis aftercontractions were manifest in 8 out of 10 and 1 out of 5 cells in the presence of an alpha 1-adrenergic agonist and in control, respectively (p <0.001). EIPA abolished the occurrence of after contractions. Thus, the arrhythmogenicity of alpha 1-adrenergic stimulation depends on activation of Na+/H+ exchanger.
| Original language | Italian |
|---|---|
| Pages (from-to) | 569-571 |
| Number of pages | 3 |
| Journal | Cardiologia |
| Volume | 37 |
| Issue number | 8 |
| Publication status | Published - Aug 1992 |
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ASJC Scopus subject areas
- Cardiology and Cardiovascular Medicine
Cite this
Aritmogenicità della stimolazione alpha 1-adrenergica dopo acidosi miocardica. / Gambassi, G.; Carbonin, P.; Capogrossi, M. C.
In: Cardiologia, Vol. 37, No. 8, 08.1992, p. 569-571.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Aritmogenicità della stimolazione alpha 1-adrenergica dopo acidosi miocardica.
AU - Gambassi, G.
AU - Carbonin, P.
AU - Capogrossi, M. C.
PY - 1992/8
Y1 - 1992/8
N2 - Ischemia is associated with myocardial acidosis which recovers upon reperfusion. In such conditions, alpha 1-adrenergic stimulation is arrhythmogenic. We used single cardiac myocytes loaded with the pH fluorescent dye, SNARF-1, to determine if a modulation of pH could explain the effect of alpha 1-adrenergic stimulation. Cells were exposed to acidosis (CO2 15%) for 15 min and then normocapnia restored. During acidosis, alpha 1-adrenergic stimulation caused an increase in pH which was abolished by blocking Na+/H+ exchange with ethylisopropylamiloride (EIPA). After removal of acidosis aftercontractions were manifest in 8 out of 10 and 1 out of 5 cells in the presence of an alpha 1-adrenergic agonist and in control, respectively (p <0.001). EIPA abolished the occurrence of after contractions. Thus, the arrhythmogenicity of alpha 1-adrenergic stimulation depends on activation of Na+/H+ exchanger.
AB - Ischemia is associated with myocardial acidosis which recovers upon reperfusion. In such conditions, alpha 1-adrenergic stimulation is arrhythmogenic. We used single cardiac myocytes loaded with the pH fluorescent dye, SNARF-1, to determine if a modulation of pH could explain the effect of alpha 1-adrenergic stimulation. Cells were exposed to acidosis (CO2 15%) for 15 min and then normocapnia restored. During acidosis, alpha 1-adrenergic stimulation caused an increase in pH which was abolished by blocking Na+/H+ exchange with ethylisopropylamiloride (EIPA). After removal of acidosis aftercontractions were manifest in 8 out of 10 and 1 out of 5 cells in the presence of an alpha 1-adrenergic agonist and in control, respectively (p <0.001). EIPA abolished the occurrence of after contractions. Thus, the arrhythmogenicity of alpha 1-adrenergic stimulation depends on activation of Na+/H+ exchanger.
UR - http://www.scopus.com/inward/record.url?scp=0026907483&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0026907483&partnerID=8YFLogxK
M3 - Articolo
C2 - 1336711
AN - SCOPUS:0026907483
VL - 37
SP - 569
EP - 571
JO - Cardiologia (Rome, Italy)
JF - Cardiologia (Rome, Italy)
SN - 0393-1978
IS - 8
ER -