The artificial zinc finger protein 'Blues' binds the enhancer of the fibroblast growth factor 4 and represses transcription

V. Libri, A. Onori, M. Fanciulli, C. Passananti, N. Corbi

Research output: Contribution to journalArticle

Abstract

The design of novel genes encoding artificial transcription factors represents a powerful tool in biotechnology and medicine. We have engineered a new zinc finger-based transcription factor, named Blues, able to bind and possibly to modify the expression of fibroblast growth factor 4 (FGF-4, K-fgf), originally identified as an oncogene. Blues encodes a three zinc finger peptide and was constructed to target the 9 bp DNA sequence: 5′-GTT-TGG-ATG- 3′, internal to the murine FGF-4 enhancer, in proximity of Sox-2 and Oct-3 DNA binding sites. Our final aim is to generate a model system based on artificial zinc finger genes to study the biological role of FGF-4 during development and tumorigenesis.

Original languageEnglish
Pages (from-to)75-80
Number of pages6
JournalFEBS Letters
Volume560
Issue number1-3
DOIs
Publication statusPublished - Feb 27 2004

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Keywords

  • Artificial transcription factor
  • Fibroblast growth factor 4
  • Oncogene
  • Recognition code
  • Zinc finger

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

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