The ataxia related G1107D mutation of the plasma membrane Ca(2+) ATPase isoform 3 affects its interplay with calmodulin and the autoinhibition process

Tito Calì, Martina Frizzarin, Laura Luoni, Francesco Zonta, Sergio Pantano, Carlos Cruz, Maria Cristina Bonza, Ilenia Bertipaglia, Maria Ruzzene, Maria Ida De Michelis, Nunzio Damiano, Oriano Marin, Ginevra Zanni, Giuseppe Zanotti, Marisa Brini, Raffaele Lopreiato, Ernesto Carafoli

Research output: Contribution to journalArticle

Abstract

The plasma membrane Ca(2+) ATPases (PMCA pumps) have a long, cytosolic C-terminal regulatory region where a calmodulin-binding domain (CaM-BD) is located. Under basal conditions (low Ca(2+)), the C-terminal tail of the pump interacts with autoinhibitory sites proximal to the active center of the enzyme. In activating conditions (i.e., high Ca(2+)), Ca(2+)-bound CaM displaces the C-terminal tail from the autoinhibitory sites, restoring activity. We have recently identified a G1107D replacement within the CaM-BD of isoform 3 of the PMCA pump in a family affected by X-linked congenital cerebellar ataxia. Here, we investigate the effects of the G1107D replacement on the interplay of the mutated CaM-BD with both CaM and the pump core, by combining computational, biochemical and functional approaches. We provide evidence that the affinity of the isolated mutated CaM-BD for CaM is significantly reduced with respect to the wild type (wt) counterpart, and that the ability of CaM to activate the pump in vitro is thus decreased. Multiscale simulations support the conclusions on the detrimental effect of the mutation, indicating reduced stability of the CaM binding. We further show that the G1107D replacement impairs the autoinhibition mechanism of the PMCA3 pump as well, as the introduction of a negative charge perturbs the contacts between the CaM-BD and the pump core. Thus, the mutation affects both the ability of the pump to optimally transport Ca(2+) in the activated state, and the autoinhibition mechanism in its resting state.

Original languageEnglish
Pages (from-to)165-173
Number of pages9
JournalBiochimica et Biophysica Acta - General Subjects
Volume1863
Issue number1
DOIs
Publication statusPublished - Sep 12 2016

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Ataxia
Calmodulin
Cell membranes
Adenosine Triphosphatases
Protein Isoforms
Cell Membrane
Pumps
Mutation
Cerebellar Ataxia
Nucleic Acid Regulatory Sequences
Enzymes

Keywords

  • Journal Article

Cite this

The ataxia related G1107D mutation of the plasma membrane Ca(2+) ATPase isoform 3 affects its interplay with calmodulin and the autoinhibition process. / Calì, Tito; Frizzarin, Martina; Luoni, Laura; Zonta, Francesco; Pantano, Sergio; Cruz, Carlos; Bonza, Maria Cristina; Bertipaglia, Ilenia; Ruzzene, Maria; De Michelis, Maria Ida; Damiano, Nunzio; Marin, Oriano; Zanni, Ginevra; Zanotti, Giuseppe; Brini, Marisa; Lopreiato, Raffaele; Carafoli, Ernesto.

In: Biochimica et Biophysica Acta - General Subjects, Vol. 1863, No. 1, 12.09.2016, p. 165-173.

Research output: Contribution to journalArticle

Calì, T, Frizzarin, M, Luoni, L, Zonta, F, Pantano, S, Cruz, C, Bonza, MC, Bertipaglia, I, Ruzzene, M, De Michelis, MI, Damiano, N, Marin, O, Zanni, G, Zanotti, G, Brini, M, Lopreiato, R & Carafoli, E 2016, 'The ataxia related G1107D mutation of the plasma membrane Ca(2+) ATPase isoform 3 affects its interplay with calmodulin and the autoinhibition process', Biochimica et Biophysica Acta - General Subjects, vol. 1863, no. 1, pp. 165-173. https://doi.org/10.1016/j.bbadis.2016.09.007
Calì, Tito ; Frizzarin, Martina ; Luoni, Laura ; Zonta, Francesco ; Pantano, Sergio ; Cruz, Carlos ; Bonza, Maria Cristina ; Bertipaglia, Ilenia ; Ruzzene, Maria ; De Michelis, Maria Ida ; Damiano, Nunzio ; Marin, Oriano ; Zanni, Ginevra ; Zanotti, Giuseppe ; Brini, Marisa ; Lopreiato, Raffaele ; Carafoli, Ernesto. / The ataxia related G1107D mutation of the plasma membrane Ca(2+) ATPase isoform 3 affects its interplay with calmodulin and the autoinhibition process. In: Biochimica et Biophysica Acta - General Subjects. 2016 ; Vol. 1863, No. 1. pp. 165-173.
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T1 - The ataxia related G1107D mutation of the plasma membrane Ca(2+) ATPase isoform 3 affects its interplay with calmodulin and the autoinhibition process

AU - Calì, Tito

AU - Frizzarin, Martina

AU - Luoni, Laura

AU - Zonta, Francesco

AU - Pantano, Sergio

AU - Cruz, Carlos

AU - Bonza, Maria Cristina

AU - Bertipaglia, Ilenia

AU - Ruzzene, Maria

AU - De Michelis, Maria Ida

AU - Damiano, Nunzio

AU - Marin, Oriano

AU - Zanni, Ginevra

AU - Zanotti, Giuseppe

AU - Brini, Marisa

AU - Lopreiato, Raffaele

AU - Carafoli, Ernesto

N1 - Copyright © 2016. Published by Elsevier B.V.

PY - 2016/9/12

Y1 - 2016/9/12

N2 - The plasma membrane Ca(2+) ATPases (PMCA pumps) have a long, cytosolic C-terminal regulatory region where a calmodulin-binding domain (CaM-BD) is located. Under basal conditions (low Ca(2+)), the C-terminal tail of the pump interacts with autoinhibitory sites proximal to the active center of the enzyme. In activating conditions (i.e., high Ca(2+)), Ca(2+)-bound CaM displaces the C-terminal tail from the autoinhibitory sites, restoring activity. We have recently identified a G1107D replacement within the CaM-BD of isoform 3 of the PMCA pump in a family affected by X-linked congenital cerebellar ataxia. Here, we investigate the effects of the G1107D replacement on the interplay of the mutated CaM-BD with both CaM and the pump core, by combining computational, biochemical and functional approaches. We provide evidence that the affinity of the isolated mutated CaM-BD for CaM is significantly reduced with respect to the wild type (wt) counterpart, and that the ability of CaM to activate the pump in vitro is thus decreased. Multiscale simulations support the conclusions on the detrimental effect of the mutation, indicating reduced stability of the CaM binding. We further show that the G1107D replacement impairs the autoinhibition mechanism of the PMCA3 pump as well, as the introduction of a negative charge perturbs the contacts between the CaM-BD and the pump core. Thus, the mutation affects both the ability of the pump to optimally transport Ca(2+) in the activated state, and the autoinhibition mechanism in its resting state.

AB - The plasma membrane Ca(2+) ATPases (PMCA pumps) have a long, cytosolic C-terminal regulatory region where a calmodulin-binding domain (CaM-BD) is located. Under basal conditions (low Ca(2+)), the C-terminal tail of the pump interacts with autoinhibitory sites proximal to the active center of the enzyme. In activating conditions (i.e., high Ca(2+)), Ca(2+)-bound CaM displaces the C-terminal tail from the autoinhibitory sites, restoring activity. We have recently identified a G1107D replacement within the CaM-BD of isoform 3 of the PMCA pump in a family affected by X-linked congenital cerebellar ataxia. Here, we investigate the effects of the G1107D replacement on the interplay of the mutated CaM-BD with both CaM and the pump core, by combining computational, biochemical and functional approaches. We provide evidence that the affinity of the isolated mutated CaM-BD for CaM is significantly reduced with respect to the wild type (wt) counterpart, and that the ability of CaM to activate the pump in vitro is thus decreased. Multiscale simulations support the conclusions on the detrimental effect of the mutation, indicating reduced stability of the CaM binding. We further show that the G1107D replacement impairs the autoinhibition mechanism of the PMCA3 pump as well, as the introduction of a negative charge perturbs the contacts between the CaM-BD and the pump core. Thus, the mutation affects both the ability of the pump to optimally transport Ca(2+) in the activated state, and the autoinhibition mechanism in its resting state.

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