The autonomous growth of human papillomavirus type 16-immortalized keratinocytes is related to the endothelin-1 autocrine loop

Aldo Venuti, Maria Luisa Marcante, Silvio Flamini, Valeriana Di Castro, Anna Bagnato

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Some human papillomaviruses (HPVs) such as HPV type 16 (HPV16) and HPV18 are involved in cervical carcinoma, and they can immortalize and transform keratinocytes. Endothelin-1 (ET-1) is produced in keratinocytes and has been shown to act through ETA receptors as an autocrine growth factor for keratinocytes. This study examines whether HPV16 alters the ET-1-mediated autocrine loop in human keratinocytes, providing a selective growth advantage for transformed cells. ET-1 is released in similar amounts from normal and HPV-transfected keratinocytes. All HPV-transfected cell lines express high- affinity ET(A) receptors. A two-fold increase in ET-1 binding sites is present in HPV16-immortalized keratinocytes, and this effect seems to be linked to the overexpression of mRNA for this receptor rather than to differences in the surface/internalized ratio of the receptors. ET-1 induces significant increases in [3H] thymidine incorporation and cell proliferation. Furthermore, HPV-transfected keratinocytes can proliferate in the absence of any growth factor added to the growth medium, and the ET(A) receptor antagonist BQ123 prevents this proliferation. These data suggest a new mechanism in the growth control of HPV-transformed cells mediated by the upregulation of ET-1 autocrine loop.

Original languageEnglish
Pages (from-to)6898-6904
Number of pages7
JournalJournal of Virology
Volume71
Issue number9
Publication statusPublished - 1997

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endothelins
human growth
Human papillomavirus 16
Papillomaviridae
keratinocytes
Endothelin-1
Keratinocytes
Growth
receptors
growth factors
Fibroblast Growth Factor 7
uterine cervical neoplasms
Thymidine
thymidine
Intercellular Signaling Peptides and Proteins
Up-Regulation
Binding Sites
binding sites
Cell Proliferation
antagonists

ASJC Scopus subject areas

  • Immunology

Cite this

The autonomous growth of human papillomavirus type 16-immortalized keratinocytes is related to the endothelin-1 autocrine loop. / Venuti, Aldo; Marcante, Maria Luisa; Flamini, Silvio; Di Castro, Valeriana; Bagnato, Anna.

In: Journal of Virology, Vol. 71, No. 9, 1997, p. 6898-6904.

Research output: Contribution to journalArticle

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