TY - JOUR
T1 - The avidin-biotin peroxidase complex (ABCPx) in skin immunoelectron microscopy
AU - Berti, E.
AU - Monti, M.
AU - Cavicchini, S.
AU - Caputo, R.
PY - 1983/4
Y1 - 1983/4
N2 - The interaction between avidin and biotin can be exploited in immunoperoxidase techniques in three main ways: the labeled avidin-biotin technique (LAB); the bridge avidin-biotin technique (BRAB); the performed avidin-biotin complex (ABCPx). In a comparative study of these three methods and the peroxidase antiperoxidase method using serial dilutions of antinuclear positive serum, the ABCPx method yielded the most intense staining. For this reason, the ABCPx method has been applied in immunoelectron microscopy (IEM) techniques on normal and pathological skin employing monoclonal. OKT4, OKT6, and OKT8 antibodies to detect target membrane antigens. The authors notes that the ANCPx method allows strong staining both in normal and pathological skin and improves tissue preservation.
AB - The interaction between avidin and biotin can be exploited in immunoperoxidase techniques in three main ways: the labeled avidin-biotin technique (LAB); the bridge avidin-biotin technique (BRAB); the performed avidin-biotin complex (ABCPx). In a comparative study of these three methods and the peroxidase antiperoxidase method using serial dilutions of antinuclear positive serum, the ABCPx method yielded the most intense staining. For this reason, the ABCPx method has been applied in immunoelectron microscopy (IEM) techniques on normal and pathological skin employing monoclonal. OKT4, OKT6, and OKT8 antibodies to detect target membrane antigens. The authors notes that the ANCPx method allows strong staining both in normal and pathological skin and improves tissue preservation.
KW - Avidin-biotin complex
KW - Immunoelectron microscopy
KW - Monoclonal antibodies
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U2 - 10.1007/BF00412890
DO - 10.1007/BF00412890
M3 - Article
C2 - 6347097
AN - SCOPUS:0020583551
VL - 275
SP - 134
EP - 138
JO - Archives of Dermatological Research
JF - Archives of Dermatological Research
SN - 0340-3696
IS - 2
ER -