The B-oligomer of pertussis toxin inhibits human immunodeficiency virus type 1 replication at multiple stages

M. Alfano, T. Pushkarsky, G. Poli, M. Bukrinsky

Research output: Contribution to journalArticle

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Abstract

We have recently demonstrated that the binding subunit (B-oligomer) of pertussis toxin (PTX-B) deactivates CCR5 and inhibits entry of R5 human immunodeficiency virus type 1 (HIV-1) strains in activated primary T lymphocytes (M. Alfano et al., J. Exp. Med. 190:597-605, 1999). We now present evidence that PTX-B also affects a postentry step of HIV-1 replication. While PTX-B inhibited fusion induced by R5 but not that induced by X4 envelopes, it blocked infection of T cells with recombinant HIV-1 particles pseudotyped with R5, X4, and even murine leukemia virus or vesicular stomatitis virus envelopes. It also suppressed HIV-1 RNA synthesis in cultures of infected peripheral blood mononuclear cells when new infections had been inhibited by zidovudine, and it reduced Tat-dependent expression of the luciferase reporter gene controlled by the HIV-1 long terminal repeat (LTR). Surprisingly, PTX-B did not affect expression from the cytomegalovirus promoter, nor did it reduce the basal (Tat-independent) expression from the LTR promoter. These results indicate that PTX-B inhibits HIV-1 infection at both the entry and the postentry stages of viral replication, with the postentry activity specifically affecting transcription or stability of Tat-stimulated HIV-1 mRNAs.

Original languageEnglish
Pages (from-to)8767-8770
Number of pages4
JournalJournal of Virology
Volume74
Issue number18
DOIs
Publication statusPublished - 2000

Fingerprint

pertussis toxin
Pertussis Toxin
Virus Replication
Human immunodeficiency virus 1
HIV-1
terminal repeat sequences
T-lymphocytes
promoter regions
Murine leukemia virus
HIV Long Terminal Repeat
infection
Vesiculovirus
T-Lymphocytes
Cytomegalovirus
Murine Leukemia Viruses
Vesicular Stomatitis
Terminal Repeat Sequences
Zidovudine
Virus Diseases
luciferase

ASJC Scopus subject areas

  • Immunology

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The B-oligomer of pertussis toxin inhibits human immunodeficiency virus type 1 replication at multiple stages. / Alfano, M.; Pushkarsky, T.; Poli, G.; Bukrinsky, M.

In: Journal of Virology, Vol. 74, No. 18, 2000, p. 8767-8770.

Research output: Contribution to journalArticle

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