The B subunit of the CAAT-binding factor NFY binds the central segment of the co-activator p300

Maria Concetta Faniello, Maria Assunta Bevilacqua, GianLuigi Condorelli, Benoit De Crombrugghe, Sankar N. Maity, Vittorio Enrico Avvedimento, Filiberto Cimino, Francesco Costanzo

Research output: Contribution to journalArticlepeer-review

Abstract

We report that the heterotrimeric transcription factor NFY or 'CAAT- binding factor' binds the -60 region of the human H ferritin promoter, the B site. DNA binding analysis with specific antibodies demonstrates that NFY/B/C subunits tightly bind this site and that NFY/C subunit is masked in vivo by binding with other protein(s). NFY binds the co-activator p300. Specifically, the NFY/B subunit interacts with the central segment of p300 in vivo and in vitro. cAMP substantially increases the formation of the NFY·p300 complex. Taken together these data provide a general model of cAMP induction of non- CRE-containing promoters and suggest that the NFY-B·p300 complex is located at the 5' end of the promoter and the NFY-B·C·TFIIB on the 3' end toward the transcription start site.

Original languageEnglish
Pages (from-to)7623-7626
Number of pages4
JournalJournal of Biological Chemistry
Volume274
Issue number12
DOIs
Publication statusPublished - Mar 19 1999

ASJC Scopus subject areas

  • Biochemistry

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