The basement membrane protein BM-600/nicein codistributes with kalinin and the integrin α6β4 in human cultured keratinocytes

Pier Carlo Marchisio, Ottavio Cremona, Paola Savoia, Graziella Pellegrini, Jean Paul Ortonne, Patrick Verrando, Robert E. Burgeson, Ranieri Cancedda, Michele De Luca

Research output: Contribution to journalArticle

44 Citations (Scopus)

Abstract

The observation is reported that in low-passage human keratinocyte colonies cultured under conditions that allow full epidermal differentiation (i) the basement membrane protein BM-600/nicein, identified by the mAb GB3, is codistributed with laminin and collagen type IV as well as with the bullous pemphigoid antigen in footprints deposited by growing and migrating colonies; (ii) the integrin heterodimer α6β4 is codistributed with the same molecules suggesting its spatial association with basement membrane components; (iii) the distribution pattern of α6β4 and BM-600/nicein underneath individual cells is identical and is characterized by a typical "leopard skin" pattern complementary to the distribution of submembraneous F-actin microfilament network; (iv) a rabbit polyclonal antiserum to kalinin (R4012) used in double-label immunofluorescence staining with mAb GB3 shows that this protein has the same distribution as BM-600/nicein and this suggests that they are identically located; and (v) immunoprecipitation with mAb GB3 to BM-600/nicein and BM165 to kalinin shows identical bands suggesting that nicein and kalinin represent the same molecular entity. We suggest that α6β4 displays not only an adhesive role for keratinocytes in view of its reported association to hemidesmosomes but may also be involved in organizing the molecules of the epithelial extracellular matrix, including those forming the basement membrane zone and hemidesmosomes, a function proposed for other integrins in other cellular systems.

Original languageEnglish
Pages (from-to)205-212
Number of pages8
JournalExperimental Cell Research
Volume205
Issue number2
Publication statusPublished - 1993

Fingerprint

Keratinocytes
Basement Membrane
Integrins
Membrane Proteins
Hemidesmosomes
kalinin
Panthera
Bullous Pemphigoid
Collagen Type IV
Laminin
Actin Cytoskeleton
Immunoprecipitation
Adhesives
Fluorescent Antibody Technique
Extracellular Matrix
Actins
Immune Sera
Observation
Staining and Labeling
Rabbits

ASJC Scopus subject areas

  • Cell Biology

Cite this

Marchisio, P. C., Cremona, O., Savoia, P., Pellegrini, G., Ortonne, J. P., Verrando, P., ... De Luca, M. (1993). The basement membrane protein BM-600/nicein codistributes with kalinin and the integrin α6β4 in human cultured keratinocytes. Experimental Cell Research, 205(2), 205-212.

The basement membrane protein BM-600/nicein codistributes with kalinin and the integrin α6β4 in human cultured keratinocytes. / Marchisio, Pier Carlo; Cremona, Ottavio; Savoia, Paola; Pellegrini, Graziella; Ortonne, Jean Paul; Verrando, Patrick; Burgeson, Robert E.; Cancedda, Ranieri; De Luca, Michele.

In: Experimental Cell Research, Vol. 205, No. 2, 1993, p. 205-212.

Research output: Contribution to journalArticle

Marchisio, PC, Cremona, O, Savoia, P, Pellegrini, G, Ortonne, JP, Verrando, P, Burgeson, RE, Cancedda, R & De Luca, M 1993, 'The basement membrane protein BM-600/nicein codistributes with kalinin and the integrin α6β4 in human cultured keratinocytes', Experimental Cell Research, vol. 205, no. 2, pp. 205-212.
Marchisio, Pier Carlo ; Cremona, Ottavio ; Savoia, Paola ; Pellegrini, Graziella ; Ortonne, Jean Paul ; Verrando, Patrick ; Burgeson, Robert E. ; Cancedda, Ranieri ; De Luca, Michele. / The basement membrane protein BM-600/nicein codistributes with kalinin and the integrin α6β4 in human cultured keratinocytes. In: Experimental Cell Research. 1993 ; Vol. 205, No. 2. pp. 205-212.
@article{64e384b5c72547f9800156e4436e13a8,
title = "The basement membrane protein BM-600/nicein codistributes with kalinin and the integrin α6β4 in human cultured keratinocytes",
abstract = "The observation is reported that in low-passage human keratinocyte colonies cultured under conditions that allow full epidermal differentiation (i) the basement membrane protein BM-600/nicein, identified by the mAb GB3, is codistributed with laminin and collagen type IV as well as with the bullous pemphigoid antigen in footprints deposited by growing and migrating colonies; (ii) the integrin heterodimer α6β4 is codistributed with the same molecules suggesting its spatial association with basement membrane components; (iii) the distribution pattern of α6β4 and BM-600/nicein underneath individual cells is identical and is characterized by a typical {"}leopard skin{"} pattern complementary to the distribution of submembraneous F-actin microfilament network; (iv) a rabbit polyclonal antiserum to kalinin (R4012) used in double-label immunofluorescence staining with mAb GB3 shows that this protein has the same distribution as BM-600/nicein and this suggests that they are identically located; and (v) immunoprecipitation with mAb GB3 to BM-600/nicein and BM165 to kalinin shows identical bands suggesting that nicein and kalinin represent the same molecular entity. We suggest that α6β4 displays not only an adhesive role for keratinocytes in view of its reported association to hemidesmosomes but may also be involved in organizing the molecules of the epithelial extracellular matrix, including those forming the basement membrane zone and hemidesmosomes, a function proposed for other integrins in other cellular systems.",
author = "Marchisio, {Pier Carlo} and Ottavio Cremona and Paola Savoia and Graziella Pellegrini and Ortonne, {Jean Paul} and Patrick Verrando and Burgeson, {Robert E.} and Ranieri Cancedda and {De Luca}, Michele",
year = "1993",
language = "English",
volume = "205",
pages = "205--212",
journal = "Experimental Cell Research",
issn = "0014-4827",
publisher = "Academic Press Inc.",
number = "2",

}

TY - JOUR

T1 - The basement membrane protein BM-600/nicein codistributes with kalinin and the integrin α6β4 in human cultured keratinocytes

AU - Marchisio, Pier Carlo

AU - Cremona, Ottavio

AU - Savoia, Paola

AU - Pellegrini, Graziella

AU - Ortonne, Jean Paul

AU - Verrando, Patrick

AU - Burgeson, Robert E.

AU - Cancedda, Ranieri

AU - De Luca, Michele

PY - 1993

Y1 - 1993

N2 - The observation is reported that in low-passage human keratinocyte colonies cultured under conditions that allow full epidermal differentiation (i) the basement membrane protein BM-600/nicein, identified by the mAb GB3, is codistributed with laminin and collagen type IV as well as with the bullous pemphigoid antigen in footprints deposited by growing and migrating colonies; (ii) the integrin heterodimer α6β4 is codistributed with the same molecules suggesting its spatial association with basement membrane components; (iii) the distribution pattern of α6β4 and BM-600/nicein underneath individual cells is identical and is characterized by a typical "leopard skin" pattern complementary to the distribution of submembraneous F-actin microfilament network; (iv) a rabbit polyclonal antiserum to kalinin (R4012) used in double-label immunofluorescence staining with mAb GB3 shows that this protein has the same distribution as BM-600/nicein and this suggests that they are identically located; and (v) immunoprecipitation with mAb GB3 to BM-600/nicein and BM165 to kalinin shows identical bands suggesting that nicein and kalinin represent the same molecular entity. We suggest that α6β4 displays not only an adhesive role for keratinocytes in view of its reported association to hemidesmosomes but may also be involved in organizing the molecules of the epithelial extracellular matrix, including those forming the basement membrane zone and hemidesmosomes, a function proposed for other integrins in other cellular systems.

AB - The observation is reported that in low-passage human keratinocyte colonies cultured under conditions that allow full epidermal differentiation (i) the basement membrane protein BM-600/nicein, identified by the mAb GB3, is codistributed with laminin and collagen type IV as well as with the bullous pemphigoid antigen in footprints deposited by growing and migrating colonies; (ii) the integrin heterodimer α6β4 is codistributed with the same molecules suggesting its spatial association with basement membrane components; (iii) the distribution pattern of α6β4 and BM-600/nicein underneath individual cells is identical and is characterized by a typical "leopard skin" pattern complementary to the distribution of submembraneous F-actin microfilament network; (iv) a rabbit polyclonal antiserum to kalinin (R4012) used in double-label immunofluorescence staining with mAb GB3 shows that this protein has the same distribution as BM-600/nicein and this suggests that they are identically located; and (v) immunoprecipitation with mAb GB3 to BM-600/nicein and BM165 to kalinin shows identical bands suggesting that nicein and kalinin represent the same molecular entity. We suggest that α6β4 displays not only an adhesive role for keratinocytes in view of its reported association to hemidesmosomes but may also be involved in organizing the molecules of the epithelial extracellular matrix, including those forming the basement membrane zone and hemidesmosomes, a function proposed for other integrins in other cellular systems.

UR - http://www.scopus.com/inward/record.url?scp=0027217454&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0027217454&partnerID=8YFLogxK

M3 - Article

C2 - 8482332

AN - SCOPUS:0027217454

VL - 205

SP - 205

EP - 212

JO - Experimental Cell Research

JF - Experimental Cell Research

SN - 0014-4827

IS - 2

ER -