The benefits of analysing complete mitochondrial genomes: Deep insights into the phylogeny and population structure of Echinococcus granulosus sensu lato genotypes G6 and G7

Teivi Laurimäe, Liina Kinkar, Thomas Romig, Rihab A. Omer, Adriano Casulli, Gérald Umhang, Robin B. Gasser, Abdul Jabbar, Mitra Sharbatkhori, Hossein Mirhendi, Francisco Ponce-Gordo, Lorena E. Lazzarini, Silvia V. Soriano, Antonio Varcasia, Mohammad Rostami Nejad, Vanessa Andresiuk, Pablo Maravilla, Luis Miguel González, Monika Dybicz, Jakub GaworMindaugas Šarkūnas, Viliam Šnábel, Tetiana Kuzmina, Urmas Saarma

Research output: Contribution to journalArticle

Abstract

Cystic echinococcosis (CE) is a zoonotic disease caused by the larval stage of the species complex Echinococcus granulosus sensu lato. Within this complex, genotypes G6 and G7 have been frequently associated with human CE worldwide. Previous studies exploring the genetic variability and phylogeography of genotypes G6 and G7 have been based on relatively short mtDNA sequences, and the resolution of these studies has often been low. Moreover, using short sequences, the distinction between G6 and G7 has in some cases remained challenging. The aim here was to sequence complete mitochondrial genomes (mitogenomes) to obtain deeper insight into the genetic diversity, phylogeny and population structure of genotypes G6 and G7. We sequenced complete mitogenomes of 94 samples collected from 15 different countries worldwide. The results demonstrated that (i) genotypes G6 and G7 can be clearly distinguished when mitogenome sequences are used; (ii) G7 is represented by two major haplogroups, G7a and G7b, the latter being specific to islands of Corsica and Sardinia; (iii) intensive animal trade, but also geographical isolation, have likely had the largest impact on shaping the genetic structure and distribution of genotypes G6 and G7. In addition, we found phylogenetically highly divergent haplotype from Mongolia (Gmon), which had a higher affinity to G6.

Original languageEnglish
Pages (from-to)85-94
Number of pages10
JournalInfection, Genetics and Evolution
Volume64
DOIs
Publication statusPublished - Oct 1 2018

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Keywords

  • Cystic echinococcosis
  • Genotype G6
  • Genotype G7
  • Hydatid disease
  • Mitochondrial genome
  • Zoonosis

ASJC Scopus subject areas

  • Microbiology
  • Ecology, Evolution, Behavior and Systematics
  • Molecular Biology
  • Genetics
  • Microbiology (medical)
  • Infectious Diseases

Cite this

Laurimäe, T., Kinkar, L., Romig, T., Omer, R. A., Casulli, A., Umhang, G., Gasser, R. B., Jabbar, A., Sharbatkhori, M., Mirhendi, H., Ponce-Gordo, F., Lazzarini, L. E., Soriano, S. V., Varcasia, A., Rostami Nejad, M., Andresiuk, V., Maravilla, P., González, L. M., Dybicz, M., ... Saarma, U. (2018). The benefits of analysing complete mitochondrial genomes: Deep insights into the phylogeny and population structure of Echinococcus granulosus sensu lato genotypes G6 and G7. Infection, Genetics and Evolution, 64, 85-94. https://doi.org/10.1016/j.meegid.2018.06.016