The bioenergetics of mitochondria after cryopreservation

Barry J. Fuller, Alessandro Rubinacci, Karel Geboes, William De Loecker

Research output: Contribution to journalArticlepeer-review

Abstract

The functional characteristics of rat liver mitochondria after cryopreservation with and without the addition of the cryoprotectant dimethyl sulfoxide (Me2SO) were evaluated. As criteria of functional integrity, polarographic measurements of substrate-linked oxygen consumption and luminescent assay of adenosine triphosphate (ATP) synthesis were considered before and after cryopreservation. The results demonstrated that mitochondrial damage after freezing was indicated by the polarographic studies but was not evident when ATP synthesis was considered. Me2SO present during cryopreservation was partially protective for mitochondrial substrate-linked oxygen consumption; however, simple exposure to and dilution from Me2SO effected some changes in mitochondrial function.

Original languageEnglish
Pages (from-to)333-340
Number of pages8
JournalCryobiology
Volume26
Issue number4
DOIs
Publication statusPublished - 1989

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)

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