The Blockade of K+-ATP Channels has Neuroprotective Effects in an In Vitro Model of Brain Ischemia

Robert Nisticò, Silvia Piccirilli, L. Sebastianelli, Giuseppe Nisticò, G. Bernardi, N. B. Mercuri

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

There is a common belief that the opening of K+-ATP channels during an ischemic episode has protective effects on neuronal functions by inducing a reduction in energy consumption. However, recent studies have also proposed that activation of these channels might have deleterious effects on cell's survival possibly after a stroke or during long-lasting neurodegenerative processes. Considering these contrasting results, we have used a hippocampal in vitro slice preparation in order to investigate the possible effects of K+-ATP channel blockers on the electrophysiological and morphological changes induced by a transient episode of ischemia (oxygen and glucose deprivation) on CA1 pyramidal neurons. Therefore, we found that tolbutamide and glibenclamide, both nonselective K+-ATP channel blockers, produce neuroprotective effects against in vitro ischemia. Interestingly, the mitochondrial K+-ATP channel blocker 5-hydroxydecanoate and various K+ channel blockers did not exert neuroprotection. Our results are consistent with the concept that a decreased activity of the plasmalemmal K+-ATP conductances may have a protective effect during episodes of transient cerebral ischemia.

Original languageEnglish
Pages (from-to)383-395
Number of pages13
JournalInternational Review of Neurobiology
Volume82
DOIs
Publication statusPublished - 2007

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Neuroprotective Agents
Brain Ischemia
Adenosine Triphosphate
Ischemia
Tolbutamide
Glyburide
Pyramidal Cells
Transient Ischemic Attack
Cell Survival
Stroke
Oxygen
Glucose
In Vitro Techniques

ASJC Scopus subject areas

  • Neuroscience(all)
  • Neuropsychology and Physiological Psychology
  • Physiology

Cite this

The Blockade of K+-ATP Channels has Neuroprotective Effects in an In Vitro Model of Brain Ischemia. / Nisticò, Robert; Piccirilli, Silvia; Sebastianelli, L.; Nisticò, Giuseppe; Bernardi, G.; Mercuri, N. B.

In: International Review of Neurobiology, Vol. 82, 2007, p. 383-395.

Research output: Contribution to journalArticle

Nisticò, Robert ; Piccirilli, Silvia ; Sebastianelli, L. ; Nisticò, Giuseppe ; Bernardi, G. ; Mercuri, N. B. / The Blockade of K+-ATP Channels has Neuroprotective Effects in an In Vitro Model of Brain Ischemia. In: International Review of Neurobiology. 2007 ; Vol. 82. pp. 383-395.
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