We identified two types of γ/δ T-cell clones: (1) BB3+ clones, which express the disulphide-linked form of the γ/δ heterodimer (type 1 receptor) and carry rearrangements of the Cγ1 gene; and (2) δTCS1+ clones, which express the nondisulphide-linked form of the γ/δ heterodimer (type 2 receptor) and carry rearrangements of the Cγ2 gene. To determine the joining (J) and variable (V) region usage of the two receptor types. Tγ and Tδ gene rearrangements and expression were analyzed in each γ/δ T-cell clone. The results showed that the J and V regions used by the two receptor types are distinct: clones that express the type 1 receptor carry potentially functional rearrangements in JγP-Vγ9 for γ and in Jδ1-Vδ2 for δ; and clones that express the type 2 receptor had potentially functional rearrangements in Jγ2-Vγ2/Vγ4 for γ and in Jδ1-Vδ1 for δ. Therefore, two clearly different γ/δ receptors (Vγ9-JγP-Cγ1/ Vδ2-Jδ1-Cδ and Vγ2/Vγ4-Jγ2-Cγ2/Vδ1-Jδ1-Cδ) predominate in peripheral blood.
|Number of pages||6|
|Publication status||Published - Jul 15 1990|
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