The Cγ1-encoded disulphide-linked and the Cγ2-encoded nondisulphide-linked forms of the γ/δ heterodimer use different γ and δ variable regions

Marta Fagioli, Ermanno Ciccone, Cristina Bottino, Brunangelo Falini, Fausto Grignani, Alessandro Moretta, Lorenzo Moretta, Pier Giuseppe Pelicci

Research output: Contribution to journalArticlepeer-review

Abstract

We identified two types of γ/δ T-cell clones: (1) BB3+ clones, which express the disulphide-linked form of the γ/δ heterodimer (type 1 receptor) and carry rearrangements of the Cγ1 gene; and (2) δTCS1+ clones, which express the nondisulphide-linked form of the γ/δ heterodimer (type 2 receptor) and carry rearrangements of the Cγ2 gene. To determine the joining (J) and variable (V) region usage of the two receptor types. Tγ and Tδ gene rearrangements and expression were analyzed in each γ/δ T-cell clone. The results showed that the J and V regions used by the two receptor types are distinct: clones that express the type 1 receptor carry potentially functional rearrangements in JγP-Vγ9 for γ and in Jδ1-Vδ2 for δ; and clones that express the type 2 receptor had potentially functional rearrangements in Jγ2-Vγ2/Vγ4 for γ and in Jδ1-Vδ1 for δ. Therefore, two clearly different γ/δ receptors (Vγ9-JγP-Cγ1/ Vδ2-Jδ1-Cδ and Vγ2/Vγ4-Jγ2-Cγ2/Vδ1-Jδ1-Cδ) predominate in peripheral blood.

Original languageEnglish
Pages (from-to)279-284
Number of pages6
JournalBlood
Volume76
Issue number2
Publication statusPublished - Jul 15 1990

ASJC Scopus subject areas

  • Hematology

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