The cdc2-related kinase, PISSLRE, is essential for cell growth and acts in G2 phase of the cell cycle

S. Li, T. K. MacLachlan, A. De Luca, P. P. Claudio, G. Condorelli, A. Giordano

Research output: Contribution to journalArticle

Abstract

Mammalian cell cycle progression is regulated by several protein kinases that are activated by cyclically expressed proteins called cyclins. These cyclin-dependent kinases, the prototype of which is the cdc2 mitosis- promoting kinase, are known to phosphorylate substrates the modified status of which is critical for the cell to progress into sequential phases of the cycle. Recently, a new cdc2-related protein kinase has been discovered, PISSLRE, named with respect to its homology to the cdc2 PSTAIRE amino acid domain. Here we report that by using both antisense and dominant-negative mutant constructs of PISSLRE when overexpressed in U2OS cells, a growth suppression is found. Furthermore, the dominant negative forms of PISSLRE halt cell cycle progression in G2-M. Therefore, PISSLRE is essential for cellular proliferation, and its effect is exerted in G2-M. This describes the first evidence since cdc2 of a cdc2-related kinase acting through G2-M.

Original languageEnglish
Pages (from-to)3992-3995
Number of pages4
JournalCancer Research
Volume55
Issue number18
Publication statusPublished - 1995

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

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    Li, S., MacLachlan, T. K., De Luca, A., Claudio, P. P., Condorelli, G., & Giordano, A. (1995). The cdc2-related kinase, PISSLRE, is essential for cell growth and acts in G2 phase of the cell cycle. Cancer Research, 55(18), 3992-3995.