The cDNA encoding Xenopus laevis heat-shock factor 1 (XHSF1): nucleotide and deduced amino-acid sequences, and properties of the encoded protein

Donald G. Stump, Nicoletta Landsberger, Alan P. Wolffe

Research output: Contribution to journalArticlepeer-review

Abstract

We have isolated a cDNA encoding Xenopus laevis (Xl) heat-shock factor 1 (XHSF1). XHSF1, translated from the mRNA synthesized in vitro, will bind specifically to the X1 hsp70 promoter (hsp70). Microinjection of XHSF1 mRNA into Xl oocytes leads to synthesis of XHSF1 which accumulates in the nucleus and selectively activates Xl phsp70p activity at 18°C.

Original languageEnglish
Pages (from-to)207-211
Number of pages5
JournalGene
Volume160
Issue number2
DOIs
Publication statusPublished - 1995

Keywords

  • expression
  • heat-shock promoter
  • Recombinant DNA
  • transcriptional regulation

ASJC Scopus subject areas

  • Genetics

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