The chemokine receptor CCR8 mediates rescue from dexamethasone-induced apoptosis via an ERK-dependent pathway

Gaia Spinetti, Giovanni Bernardini, Grazia Camarda, Antonella Mangoni, Angela Santoni, Maurizio C. Capogrossi, Monica Napolitano

Research output: Contribution to journalArticle

Abstract

Several chemokines have been shown to regulate cellular apoptosis following discrete stimuli. It was previously demonstrated that the CC chemokine CCL1 (I-309) rescues thymic lymphoma cells from apoptosis by unknown mechanisms. The aim of our study was to characterize the role of the CC chemokine receptor 8 (CCR8), the only described receptor for CCL1, in the rescue of murine thymic lymphoma cells and murine thymocytes from dexamethasone (dex)-induced apoptosis. We show here that the CCR8-restricted agonist Kaposi sarcoma-associated herpesvirus-encoded chemokine viral macrophage-inflammatory protein-1 (vMIP-1) rescues thymic lymphoma cells from dex-induced apoptosis, similar to CCL1, and that such rescue is extracellular-regulated kinase-dependent. Although it has been hypothesized that the rescuing effect of CCL1 from apoptosis could be CCR8-mediated, here, we formally demonstrate the role of such receptor as its selective antagonist encoded by the MC148 gene of molluscum contagiosum virus MC148/vMCC-I inhibits v-MIP-1- and CCL1-induced rescue activity. In addition, CCR8 ligands inhibit dex-induced apoptosis of murine thymocytes with potential implications for thymic selection.

Original languageEnglish
Pages (from-to)201-207
Number of pages7
JournalJournal of Leukocyte Biology
Volume73
Issue number1
DOIs
Publication statusPublished - Jan 1 2003

Keywords

  • Cell death
  • Kinases
  • Thymocytes
  • Viral

ASJC Scopus subject areas

  • Cell Biology

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