The chemokine SDF-1 is a chemoattractant for human CD34+ hematopoietic progenitor cells and provides a new mechanism to explain the mobilization of CD34+ progenitors to peripheral blood

A. Aiuti, I. J. Webb, C. Bleul, T. Springer, J. C. Gutierrez-Ramos

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Abstract

Hematopoietic progenitor cells migrate in vitro and in vivo towards a gradient of the chemotactic factor stromal cell-derived factor-1 (SDF-1) produced by stromal cells. This is the first chemoattractant reported for human CD34+ progenitor cells. Concentrations of SDF-1 that elicit chemotaxis also induce a transient elevation of cytoplasmic calcium in CD34+ cells. SDF-1-induced chemotaxis is inhibited by pertussis toxin, suggesting that its signaling CD34+ cells is mediated by seven transmembrane receptors coupled to G1 proteins. CD34+ cells migrating of SDF-1 include cells with a more primitive (CD34+/CD38- or CD34+/DR-) phenotype as well as CD34+ cells phenotypically committed to the erythroid, lymphoid and myeloid lineages, including functional BFU-E, CFU-GM, and CFU- MIX progenitors. Chemotaxis of CD34+ cells in response to SDF-1 is increased by IL-3 in vitro and is lower in CD34+ progenitors from peripheral blood than in CD34+ progenitors from bone marrow, suggesting that an altered response to SDF-1 may be associated with CD34 progenitor mobilization.

Original languageEnglish
Pages (from-to)111-120
Number of pages10
JournalJournal of Experimental Medicine
Volume185
Issue number1
DOIs
Publication statusPublished - Jan 6 1997

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Chemokine CXCL12
Chemotactic Factors
Hematopoietic Stem Cells
Chemokines
Chemotaxis
Granulocyte-Macrophage Progenitor Cells
Erythroid Precursor Cells
Interleukin-3
Pertussis Toxin
Stromal Cells
Stem Cells
Bone Marrow
Calcium
Phenotype
Proteins

ASJC Scopus subject areas

  • Immunology

Cite this

The chemokine SDF-1 is a chemoattractant for human CD34+ hematopoietic progenitor cells and provides a new mechanism to explain the mobilization of CD34+ progenitors to peripheral blood. / Aiuti, A.; Webb, I. J.; Bleul, C.; Springer, T.; Gutierrez-Ramos, J. C.

In: Journal of Experimental Medicine, Vol. 185, No. 1, 06.01.1997, p. 111-120.

Research output: Contribution to journalArticle

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