The chemotactic and mitogenic effects of platelet-derived growth factor-BB on rat aorta smooth muscle cells are inhibited by basic fibroblast growth factor

A. Facchiano, F. De Marchis, E. Turchetti, F. Facchiano, M. Guglielmi, A. Denaro, R. Palumbo, M. Scoccianti, M. C. Capogrossi

Research output: Contribution to journalArticle

Abstract

In response to endovascular injury, platelet-derived growth factor-BB (PDGF-BB) and basic fibroblast growth factor (bFGF) are released locally and modulate vascular smooth muscle cells (SMC) proliferation and migration within the vascular wall. The aim of the present in vitro study was to determine how rat aorta SMC respond to the simultaneous exposure to PDGF-BB and bFGF. In a modified Boyden chamber assay bFGF exhibited a dose-dependent effect to inhibit the chemotactic action of PDGF-BB. A comparable result was observed in proliferation assays. In contrast, MIP-1 β, epidermal growth factor (EGF), fibronectin and acidic FGF (aFGF) did not inhibit the chemotactic effect of PDGF-BB. Denatured bFGF did not exert an inhibitory effect and neutralizing antibodies either to bFGF or to bFGF-receptor abolished the inhibition observed in the presence of bFGF. The role played by PDGF receptor α (PDGF-Rα) was investigated in PDGF-Rα-dominant negative-transfected SMC, by selectively blocking PDGF-BB-binding to PDGF-Rα with neomycin, by neutralizing PDGF-Rα with a monoclonal antibody and by selectively stimulating PDGF-Rα with PDGF-AA; in all cases the effect of bFGF to inhibit PDGF-BB-directed SMC migration was abolished. These in vitro studies show that bFGF significantly inhibits PDGF-BB-induced SMC migration and proliferation and that this effect is mediated by both PDGF-Rα and bFGF receptor.

Original languageEnglish
Pages (from-to)2855-2863
Number of pages9
JournalJournal of Cell Science
Volume113
Issue number16
Publication statusPublished - 2000

Keywords

  • Basic fibroblast growth factor
  • Migration
  • Platelet derived growth factor
  • Proliferation
  • Smooth muscle cell

ASJC Scopus subject areas

  • Cell Biology

Fingerprint Dive into the research topics of 'The chemotactic and mitogenic effects of platelet-derived growth factor-BB on rat aorta smooth muscle cells are inhibited by basic fibroblast growth factor'. Together they form a unique fingerprint.

  • Cite this

    Facchiano, A., De Marchis, F., Turchetti, E., Facchiano, F., Guglielmi, M., Denaro, A., Palumbo, R., Scoccianti, M., & Capogrossi, M. C. (2000). The chemotactic and mitogenic effects of platelet-derived growth factor-BB on rat aorta smooth muscle cells are inhibited by basic fibroblast growth factor. Journal of Cell Science, 113(16), 2855-2863.