The chick embryo chorioallantoic membrane (CAM) assay

Domenico Ribatti

Research output: Contribution to journalArticlepeer-review


During avian development the mesodermal layers of the allantois and chorion fuse to form the chorioallantoic membrane (CAM). This structure rapidly expands generating a rich vascular network that provides an interface for gas and waste exchange. The CAM allows to study tissue grafts, tumor growth and metastasis, drugs delivery and toxicologic analysis, and angiogenic and anti-angiogenic molecules. The CAM is relatively simple, quick, and low-cost model that allows screening of a large number of pharmacological samples in a short time; does not require administrative procedures for obtaining ethics committee approval for animal experimentation. Moreover, being naturally immunodeficient, the chick embryo may receive transplantations from different tissues and species, without immune responses.

Original languageEnglish
Pages (from-to)97-101
JournalReproductive Toxicology
Publication statusPublished - Jun 2017


  • Angiogenesis
  • Anti-angiogenesis
  • Chorioallantoic membrane
  • Tissue grafts
  • Toxicology,tumor growth

ASJC Scopus subject areas

  • Toxicology


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