The clinical and biological features of a series of immunophenotypic variant of B-CLL

Lilla Cro, Andrea Ferrario, Marta Lionetti, Francesco Bertoni, Nadia Zucal N, Lucia Nobili, Sonia Fabris, Katia Todoerti, Agostino Cortelezzi, Andrea Guffanti, Maria Goldaniga, Luigi Marcheselli, Antonino Neri, Giorgio Lambertenghi-Deliliers, Luca Baldini

Research output: Contribution to journalArticle


Objectives: To describe the clinical and biological features of a series of immunophenotypic variant of B-CLL (v-CLL) characterised by intermediate RMH score, in the absence of t(11;14)(q13;q32) in FISH analysis in comparison with a series of typical CLL. Methods: We studied the clinical and biological features of 63 cases of v-CLL and 130 cases of CLL. Results: We observed significant differences in terms of age 9/L (P <0.001), lymphocyte doubling time ≤12 months (P = 0.02), high serum β2-microglobulin levels (P <0.001) and splenomegaly (P = 0.002); CD38, CD49d, CD1c were more expressed in v-CLL, CD43 in CLL (P <0.001). IgV H mutation and trisomy 12 were more frequent in v-CLL group (P = 0.001; P <0.001); del13q14 in CLL (P = 0.008). Gene expression profiling of nine v-CLL and 60 CLL indicated that the atypical group presented a specific molecular pattern. After a median follow-up of respectively, 55 (4-196) and 60 months (6-180), 25/42 patients with v-CLL (48%) and 55/93 patients with CLL (59%) were treated. Time to treatment was significantly shorter in IgV H-mutated v-CLL vs. mutated CLL (P = 0.006). The median overall survival was worse in v-CLL-mutated cases (P = 0.062). Conclusion: v-CLL should be identified and dealt with separately from classic CLL. In particular, the prognostic markers that are routinely used to characterise classical B-CLL should not be interpreted as having the same meaning.

Original languageEnglish
Pages (from-to)120-129
Number of pages10
JournalEuropean Journal of Haematology
Issue number2
Publication statusPublished - Aug 2010



  • B-cell chronic lymphocytic leukaemia
  • FISH analysis
  • IgV gene mutations
  • immunophenotype
  • ZAP-70 expression

ASJC Scopus subject areas

  • Hematology

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