TY - JOUR
T1 - The clinical and biological significance of MIR-224 expression in colorectal cancer metastasis
AU - Ling, Hui
AU - Pickard, Karen
AU - Ivan, Cristina
AU - Isella, Claudio
AU - Ikuo, Mariko
AU - Mitter, Richard
AU - Spizzo, Riccardo
AU - Bullock, Marc D.
AU - Braicu, Cornelia
AU - Pileczki, Valentina
AU - Vincent, Kimberly
AU - Pichler, Martin
AU - Stiegelbauer, Verena
AU - Hoefler, Gerald
AU - Almeida, Maria I.
AU - Hsiao, Annie
AU - Zhang, Xinna
AU - Primrose, John N.
AU - Packham, Graham K.
AU - Liu, Kevin
AU - Bojja, Krishna
AU - Gafà, Roberta
AU - Xiao, Lianchun
AU - Rossi, Simona
AU - Song, Jian H.
AU - Vannini, Ivan
AU - Fanini, Francesca
AU - Kopetz, Scott
AU - Zweidler-McKay, Patrick
AU - Wang, Xuemei
AU - Ionescu, Calin
AU - Irimie, Alexandru
AU - Fabbri, Muller
AU - Lanza, Giovanni
AU - Hamilton, Stanley R.
AU - Berindan-Neagoe, Ioana
AU - Medico, Enzo
AU - Mirnezami, Alex H.
AU - Calin, George A.
AU - Nicoloso, Milena S.
PY - 2015/3/24
Y1 - 2015/3/24
N2 - Objective MicroRNA (miRNA) expression profile can be used as prognostic marker for human cancers. We aim to explore the significance of miRNAs in colorectal cancer (CRC) metastasis. Design We performed miRNA microarrays using primary CRC tissues from patients with and without metastasis, and validated selected candidates in 85 CRC samples by quantitative real-time PCR (qRT-PCR). We tested metastatic activity of selected miRNAs and identified miRNA targets by prediction algorithms, qRT-PCR, western blot and luciferase assays. Clinical outcomes were analysed in six sets of CRC cases (n=449), including The Cancer Genome Atlas (TCGA) consortium and correlated with miR-224 status. We used the Kaplan-Meier method and log-rank test to assess the difference in survival between patients with low or high levels of miR-224 expression. Results MiR-224 expression increases consistently with tumour burden and microsatellite stable status, and miR-224 enhances CRC metastasis in vitro and in vivo. We identified SMAD4 as a miR-224 target and observed negative correlation (Spearman Rs=-0.44, p
AB - Objective MicroRNA (miRNA) expression profile can be used as prognostic marker for human cancers. We aim to explore the significance of miRNAs in colorectal cancer (CRC) metastasis. Design We performed miRNA microarrays using primary CRC tissues from patients with and without metastasis, and validated selected candidates in 85 CRC samples by quantitative real-time PCR (qRT-PCR). We tested metastatic activity of selected miRNAs and identified miRNA targets by prediction algorithms, qRT-PCR, western blot and luciferase assays. Clinical outcomes were analysed in six sets of CRC cases (n=449), including The Cancer Genome Atlas (TCGA) consortium and correlated with miR-224 status. We used the Kaplan-Meier method and log-rank test to assess the difference in survival between patients with low or high levels of miR-224 expression. Results MiR-224 expression increases consistently with tumour burden and microsatellite stable status, and miR-224 enhances CRC metastasis in vitro and in vivo. We identified SMAD4 as a miR-224 target and observed negative correlation (Spearman Rs=-0.44, p
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U2 - 10.1136/gutjnl-2015-309372
DO - 10.1136/gutjnl-2015-309372
M3 - Article
AN - SCOPUS:84929589715
JO - Gut
JF - Gut
SN - 0017-5749
ER -