TY - JOUR
T1 - The clinical predictivity of biomarkers of stage III-IV epithelial ovarian cancer in a prospective randomized treatment protocol
AU - Silvestrini, Rosella
AU - Daidone, Maria Grazia
AU - Veneroni, Silvia
AU - Benini, Elvira
AU - Scarfone, Giovanna
AU - Zanaboni, Flavia
AU - Villa, Antonella
AU - Presti, Mauro
AU - Danese, Saverio
AU - Bolis, Giorgio
PY - 1998/1/1
Y1 - 1998/1/1
N2 - BACKGROUND. The aim of this study was to define the clinical relevance of functional biomarkers, prospectively assessed in a randomized clinical protocol, in patients with Stage III-IV epithelial ovarian cancer. The protocol compared cisplatin with polychemotherapy that included cisplatin and cyclophosphamide. METHODS. In a subset of 168 patients with invasive epithelial ovarian cancer cell proliferation was determined by the 3H- thymidine labeling index, DNA ploidy was assessed by flow cytometry, and the expression of p53, bcl-2, and glutathione S-transferase-π (GST-π) was evaluated by immunohistochemistry using the antibodies PAb1801, anti-bcl-2, and GST-pi, respectively. RESULTS. Cell proliferation, DNA ploidy, and the expression of p53, bcl-2, and GST-π were generally unrelated to one another and unrelated to clinicopathologic features, except for an association between DNA ploidy and the rate of cell proliferation. All biologic variables except bcl-2 were slightly related to tumor grade. DNA ploidy emerged as a predictor of clinical complete response and 3-year overall survival, regardless of treatment type or residual disease. Conversely, except for a favorable outcome for patients with tumors not expressing bcl-2 who were treated with cisplatin, no definitive patterns of predictivity for short term or long term clinical outcomes were observed for the other biomarkers studied. CONCLUSIONS. DNA ploidy appears to be the most clinically relevant biomarker for epithelial ovarian cancer. More information is needed w understand the rote of the other markers studied in this tumor type.
AB - BACKGROUND. The aim of this study was to define the clinical relevance of functional biomarkers, prospectively assessed in a randomized clinical protocol, in patients with Stage III-IV epithelial ovarian cancer. The protocol compared cisplatin with polychemotherapy that included cisplatin and cyclophosphamide. METHODS. In a subset of 168 patients with invasive epithelial ovarian cancer cell proliferation was determined by the 3H- thymidine labeling index, DNA ploidy was assessed by flow cytometry, and the expression of p53, bcl-2, and glutathione S-transferase-π (GST-π) was evaluated by immunohistochemistry using the antibodies PAb1801, anti-bcl-2, and GST-pi, respectively. RESULTS. Cell proliferation, DNA ploidy, and the expression of p53, bcl-2, and GST-π were generally unrelated to one another and unrelated to clinicopathologic features, except for an association between DNA ploidy and the rate of cell proliferation. All biologic variables except bcl-2 were slightly related to tumor grade. DNA ploidy emerged as a predictor of clinical complete response and 3-year overall survival, regardless of treatment type or residual disease. Conversely, except for a favorable outcome for patients with tumors not expressing bcl-2 who were treated with cisplatin, no definitive patterns of predictivity for short term or long term clinical outcomes were observed for the other biomarkers studied. CONCLUSIONS. DNA ploidy appears to be the most clinically relevant biomarker for epithelial ovarian cancer. More information is needed w understand the rote of the other markers studied in this tumor type.
KW - bcl-2
KW - Cell proliferation
KW - Chemotherapy
KW - DNA ploidy
KW - Glutathione S- transferase-π
KW - Ovarian cancer
KW - P53
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U2 - 10.1002/(SICI)1097-0142(19980101)82:1<159::AID-CNCR20>3.0.CO;2-0
DO - 10.1002/(SICI)1097-0142(19980101)82:1<159::AID-CNCR20>3.0.CO;2-0
M3 - Article
C2 - 9428493
AN - SCOPUS:0031894813
VL - 82
SP - 159
EP - 167
JO - Cancer
JF - Cancer
SN - 0008-543X
IS - 1
ER -