The combined effect of dynamic conditioning followed by fluid shear stress on an EC-SMC collagen gel co-culture

Barbara Imberti; Dror Seliktar; Andrea Remuzzi; Giuseppe Remuzzi; Robert M. Nerem

The combined effect of dynamic conditioning followed by fluid shear stress on an EC-SMC collagen gel co-culture

Barbara Imberti, Dror Seliktar, Andrea Remuzzi, Giuseppe Remuzzi, Robert M. Nerem

Istituto di Ricerche Farmacologiche "Mario Negri"

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Abstract

Cell-mediated effects of cyclic circumferential stretch followed by fluid shear stress on an endothelial cell (EC) and smooth muscle cell (SMC) seeded collagen gel construct. Human aortic SMCs were seeded into a reconstituted type I collagen gel for form a tubular construct. The tubes were then exposed to 10% cyclic stretch for two days using a bioreactor system. Human aortic ECs were seeded onto the lumenal surface of the tubes and exposed to laminar flow for 48 hours. It was observed that conditioned constructs showed circumferentially oriented SMCs and collagen fibrils.

Original language	English
Title of host publication	Second Smith and Nephew International Symposium - Tissue Engineering 2000: Advances in Tissue Engineering, Biomaterials and Cell Signalling
Pages	57
Number of pages	1
Publication status	Published - 2000
Event	Second Smith and Nephew International Symposium - Tissue Engineering 2000: Advances in Tissue Engineering, Biomaterials and Cell Signalling - York, United Kingdom Duration: Jul 16 2000 → Jul 19 2000

Other

Other	Second Smith and Nephew International Symposium - Tissue Engineering 2000: Advances in Tissue Engineering, Biomaterials and Cell Signalling
Country	United Kingdom
City	York
Period	7/16/00 → 7/19/00

ASJC Scopus subject areas

Engineering(all)

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The combined effect of dynamic conditioning followed by fluid shear stress on an EC-SMC collagen gel co-culture. / Imberti, Barbara; Seliktar, Dror; Remuzzi, Andrea; Remuzzi, Giuseppe; Nerem, Robert M.

Second Smith and Nephew International Symposium - Tissue Engineering 2000: Advances in Tissue Engineering, Biomaterials and Cell Signalling. 2000. p. 57.

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Imberti, B, Seliktar, D, Remuzzi, A, Remuzzi, G & Nerem, RM 2000, The combined effect of dynamic conditioning followed by fluid shear stress on an EC-SMC collagen gel co-culture. in Second Smith and Nephew International Symposium - Tissue Engineering 2000: Advances in Tissue Engineering, Biomaterials and Cell Signalling. pp. 57, Second Smith and Nephew International Symposium - Tissue Engineering 2000: Advances in Tissue Engineering, Biomaterials and Cell Signalling, York, United Kingdom, 7/16/00.

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AB - Cell-mediated effects of cyclic circumferential stretch followed by fluid shear stress on an endothelial cell (EC) and smooth muscle cell (SMC) seeded collagen gel construct. Human aortic SMCs were seeded into a reconstituted type I collagen gel for form a tubular construct. The tubes were then exposed to 10% cyclic stretch for two days using a bioreactor system. Human aortic ECs were seeded onto the lumenal surface of the tubes and exposed to laminar flow for 48 hours. It was observed that conditioned constructs showed circumferentially oriented SMCs and collagen fibrils.

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