The Comet assay as a method of assessment of neurotoxicity: Usefulness for drugs of abuse

Giada Frenzilli, Vittoria Scarcelli, Francesco Fornai, Antonio Paparelli, Marco Nigro

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Comet assay is a quick and versatile method for assessing DNA damage in individual cells. It allows the detection of single and double DNA strand breaks, as well as the presence of alkali labile sites. DNA breaks may represent the direct effect of some damaging agent, or they may be intermediates in cellular repair. DNA strand breaks may also come from the action of free radicals generated by oxidative stress processes. The present article summarizes some data from our and other groups underlining the suitability of the Comet assay in assessing neurotoxicity and its potential in evaluating drugs of abuse-related genotoxicity.

Original languageEnglish
Title of host publicationAnnals of the New York Academy of Sciences
Pages478-481
Number of pages4
Volume1074
DOIs
Publication statusPublished - Aug 2006

Publication series

NameAnnals of the New York Academy of Sciences
Volume1074
ISSN (Print)00778923
ISSN (Electronic)17496632

Keywords

  • Brain cells
  • Comet assay
  • DNA damage
  • Drugs of abuse
  • Neurotoxicity

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

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