The condensation of chromatin in apoptotic thymocytes shows a specific structural change

Caterina Allera, Giuseppe Lazzarini, Eligio Patrone, Ingles Alberti, Paola Barboro, Paola Sanna, Antonella Melchiori, Silvio Parodi, Cecilia Balb

Research output: Contribution to journalArticle

Abstract

Chromatin condensation and DNA cleavage at internucleosomal sites have been recognized early as hallmarks of apoptosis, and it has been suggested that extensive DNA chain scission could directly result in the formation of dense chromatin bodies. Here we have shown that no causal relationship exists between DNA degradation and chromatin condensation in glucocorticoid-induced thymocyte apoptosis. The chromatin rearrangement occurred independent of as well as prior to DNA cleavage and involved a specific conformational change at the nucleosome level. In the early stages of the process, the core particles appeared to be tightly packed face-to-face in smooth 11-nm filaments that progressively folded to generate a closely woven network. The network finally collapsed, producing dense apoptotic bodies. Since trypsin digestion relaxed condensed chromatin and histone H4 underwent appreciable deacetylation in the apoptotic cell, we suggest that changes in the DNA- histone interactions represented a major modulating factor of condensation.

Original languageEnglish
Pages (from-to)10817-10822
Number of pages6
JournalJournal of Biological Chemistry
Volume272
Issue number16
DOIs
Publication statusPublished - Apr 18 1997

ASJC Scopus subject areas

  • Biochemistry

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    Allera, C., Lazzarini, G., Patrone, E., Alberti, I., Barboro, P., Sanna, P., Melchiori, A., Parodi, S., & Balb, C. (1997). The condensation of chromatin in apoptotic thymocytes shows a specific structural change. Journal of Biological Chemistry, 272(16), 10817-10822. https://doi.org/10.1074/jbc.272.16.10817