The contribution of oxidative stress in apoptosis of human-cultured astroglial cells induced by supernatants of HIV-1-infected macrophages

Vincenzo Mollace, Daniela Salvemini, Dennis P. Riley, Carolina Muscoli, Michelangelo Iannone, Teresa Granato, Laura Masuelli, Andrea Modesti, Domenicantonio Rotiroti, Robert Nisticó, Ada Bertoli, Carlo Federico Perno, Stefano Aquaro

Research output: Contribution to journalArticle

Abstract

Apoptosis of neurons and astrocytes has been found in patients undergoing AIDS dementia complex. We demonstrated that supernatants from human primary macrophages (M/M) infected by HIV-1 lead human astroglial cells to oxidative stress, as shown by elevated levels of malondialdehyde, and then to apoptosis. Electron microscopy of astrocytes shortly incubated with HIV-1-infected M/M supernatants showed apoptotic blebbing, cytoplasmic loss, and chromatin condensation. Apoptosis was antagonized by pretreating astrocytes with the nonpeptidic superoxide dismutase (SOD) mimetic M40401 but not with anti-HIV-1 compounds, thus showing that apoptosis of astrocytes driven by HIV-1-infected M/M supernatants is mainly mediated by abnormal production of superoxide anions without relationship to HIV-1 replication in such cells. Overall results support the role of oxidative stress mediated by HIV-1-infected M/M as one of the leading causes of neurodegeneration in patients with HIV-1 and suggest the use of nonpeptidic SOD mimetics to counteract HIV-1-related neurological disorders.

Original languageEnglish
Pages (from-to)65-72
Number of pages8
JournalJournal of Leukocyte Biology
Volume71
Issue number1
Publication statusPublished - 2002

Keywords

  • AIDS dementia complex
  • M40401
  • Monocytes
  • Reservoirs
  • SOD-mimetic
  • Therapy

ASJC Scopus subject areas

  • Cell Biology

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