The CpG island in the 5′ region of the G6PD gene of man and mouse

D. Toniolo, M. Filippi, R. Dono, T. Lettieri, G. Martini

Research output: Contribution to journalArticle

Abstract

The nucleotide (nt) sequence of the entire CpG island in the 5′ region of the human glucose-6-phosphate dehydrogenaseencoding gene (G6PD) and of the corresponding region in mouse was determined. In comparison to the human gene, the 5′ region of the mouse G6PD gene has highly reduced G + C and CpG dinucleotide content, but maintains the functional features of a CpG island, as it is differentially methylated on the active vs. the inactive X chromosome. In addition to the expected conservation of exons, nt sequence comparison showed that several boxes are highly conserved between the two species in the 5′ flanking DNA and in the first intron. Moreover, the conservation of the position of most CpG dinucleotides in the promoter region and in one of the upstream boxes, at about -900, gives support to the hypothesis that, in each island, specific CpGs play a major role in the regulation of gene expression.

Original languageEnglish
Pages (from-to)197-203
Number of pages7
JournalGene
Volume102
Issue number2
DOIs
Publication statusPublished - Jun 30 1991

Keywords

  • DNA methylation
  • human
  • Recombinant DNA
  • rodent
  • sequence conservation
  • X chromosome

ASJC Scopus subject areas

  • Genetics

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