The CpxRA signal transduction system of Escherichia coli: Growth-related autoactivation and control of unanticipated target operons

Peter De Wulf, Ohsuk Kwon, E. C C Lin

Research output: Contribution to journalArticle

Abstract

In Escherichia coli, the CpxRA two-component signal transduction system senses and responds to aggregated and misfolded proteins in the bacterial envelope. We show that CpxR-P (the phosphorylated form of the cognate response regulator) activates cpxRA expression in conjunction with RpoS, suggesting an involvement of the Cpx system in stationary-phase survival. Engagement of the CpxRA system in functions beyond protein management is indicated by several putative targets identified after a genomic screening for the CpxR-P recognition consensus sequence. Direct negative control of the newly identified targets motABcheAW (specifying motility and chemotaxis) and tsr (encoding the serine chemoreceptor) by CpxR-P was shown by electrophoretic mobility shift analysis and Northern hybridization. The results suggest that the CpxRA system plays a core role in an extensive stress response network in which the coordination of protein turnover and energy conservation may be the unifying element.

Original languageEnglish
Pages (from-to)6772-6778
Number of pages7
JournalJournal of Bacteriology
Volume181
Issue number21
Publication statusPublished - Nov 1999

ASJC Scopus subject areas

  • Applied Microbiology and Biotechnology
  • Immunology

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