The cyclin B1 gene is actively transcribed during mitosis in HeLa cells

Selvaggia Sciortino, Aymone Gurtner, Isabella Manni, Giulia Fontemaggi, Anup Dey, Ada Sacchi, Keiko Ozato, Giulia Piaggio

Research output: Contribution to journalArticlepeer-review

Abstract

In mammalian cells, the expression level of the cyclin B1 gene plays a critical role in the progression through mitosis. Here we demonstrate that the transcriptional activity of the human cyclin B1 promoter, as well as the rate of gene transcription, is high during mitosis. Indeed, the cyclin B1 promoter maintains an open chromatin configuration at the mitotic stage. Consistent with this, we show that the cyclin B1 promoter is occupied and bound to NF-Y during mitosis in vivo. Our results provide the first example of RNA polymerase II-dependent transcription during mitosis in mammalian cells.

Original languageEnglish
Pages (from-to)1018-1023
Number of pages6
JournalEMBO Reports
Volume2
Issue number11
DOIs
Publication statusPublished - 2001

ASJC Scopus subject areas

  • Genetics
  • Cell Biology

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