The cyclopentenone prostaglandin 15-deoxy-Δ 12,14- PGJ 2 attenuates the development of colon injury caused by dinitrobenzene sulphonic acid in the rat

Salvatore Cuzzocrea, Angela Ianaro, Nicole S. Wayman, Emanuela Mazzon, Barbara Pisano, Laura Dugo, Ivana Serraino, Rosanna Di Paola, Prabal K. Chatterjee, Massimo Di Rosa, Achille P. Caputi, Christoph Thiemermann

Research output: Contribution to journalArticle

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Abstract

1. Inflammatory bowel disease (IBD) is characterized by oxidative and nitrosative stress, leukocyte infiltration, and increased expression of the adhesion molecules intercellular adhesion molecule 1 (ICAM-1) in the colon. Recent evidence also suggests that the cyclopentenone prostaglandin (PG) 15-deoxy-Δ 12,14-PGJ 2 (15d- PGJ 2) functions as an early anti-inflammatory signal. 2. The aim of the present paper is to investigate the effects of 15d-PGJ 2 in rats subjected to experimental colitis. 3. Colitis was induced in rats by intra-colonic instillation of dinitrobenzene sulphonic acid (DNBS). 15d-PGJ 2 was administered daily as intraperitoneal injection (20 or 40 μg kg -1). On day 4, animals were sacrificed and tissues were taken for histological and biochemical analysis. 4. 15d-PGJ 2 significantly reduced the degree of haemorrhagic diarrhoea and weight loss caused by administration of DNBS. 15d-PGJ 2 also caused a substantial reduction of (i) the degree of colonic injury, (ii) the rise in myeloperoxidase (MPO) activity (mucosa), (iii) the increase in the tissue levels of malondialdehyde (MDA) and (iv) of the pro-inflammatory cytokines tumour necrosis factor-alpha (TNF-α) and interleukin-1β (IL-1β). 5. Furthermore, 15d-PGJ 2 reduced the increase in immunohistochemical staining for (i) inducible nitric oxide synthase (iNOS), (ii) nitrotyrosine and (iii) poly (ADP-ribose) polymerase (PARP), as well as (iv) the increased expression of ICAM-1 caused by DNBS in the colon. 6. Electrophoresis mobility shift assay (EMSA) of inflamed colon revealed that 15d- PGJ 2 also caused a substantial reduction of the activation of nuclear factor-kappaB (NF-κB). Furthermore, 15d-PGJ 2 stimulates the activation of heat shock protein 72 (hsp72) in the inflamed colon, as assessed by Western blot analysis. 7. In conclusion, 15d-PGJ 2 reduces the development of experimental colitis.

Original languageEnglish
Pages (from-to)678-688
Number of pages11
JournalBritish Journal of Pharmacology
Volume138
Issue number4
DOIs
Publication statusPublished - Feb 2003

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Dinitrobenzenes
Sulfonic Acids
Prostaglandins
Colon
Colitis
Wounds and Injuries
Intercellular Adhesion Molecule-1
HSP72 Heat-Shock Proteins
Poly(ADP-ribose) Polymerases
Electrophoretic Mobility Shift Assay
Nitric Oxide Synthase Type II
Intraperitoneal Injections
Malondialdehyde
Interleukin-1
Inflammatory Bowel Diseases
Peroxidase
Electrophoresis
Weight Loss
Diarrhea
Mucous Membrane

Keywords

  • 15-deoxy-Δ -PGJ
  • Colitis
  • Inflammation
  • PPAR

ASJC Scopus subject areas

  • Pharmacology

Cite this

The cyclopentenone prostaglandin 15-deoxy-Δ 12,14- PGJ 2 attenuates the development of colon injury caused by dinitrobenzene sulphonic acid in the rat. / Cuzzocrea, Salvatore; Ianaro, Angela; Wayman, Nicole S.; Mazzon, Emanuela; Pisano, Barbara; Dugo, Laura; Serraino, Ivana; Di Paola, Rosanna; Chatterjee, Prabal K.; Di Rosa, Massimo; Caputi, Achille P.; Thiemermann, Christoph.

In: British Journal of Pharmacology, Vol. 138, No. 4, 02.2003, p. 678-688.

Research output: Contribution to journalArticle

Cuzzocrea, S, Ianaro, A, Wayman, NS, Mazzon, E, Pisano, B, Dugo, L, Serraino, I, Di Paola, R, Chatterjee, PK, Di Rosa, M, Caputi, AP & Thiemermann, C 2003, 'The cyclopentenone prostaglandin 15-deoxy-Δ 12,14- PGJ 2 attenuates the development of colon injury caused by dinitrobenzene sulphonic acid in the rat', British Journal of Pharmacology, vol. 138, no. 4, pp. 678-688. https://doi.org/10.1038/sj.bjp.0705077
Cuzzocrea, Salvatore ; Ianaro, Angela ; Wayman, Nicole S. ; Mazzon, Emanuela ; Pisano, Barbara ; Dugo, Laura ; Serraino, Ivana ; Di Paola, Rosanna ; Chatterjee, Prabal K. ; Di Rosa, Massimo ; Caputi, Achille P. ; Thiemermann, Christoph. / The cyclopentenone prostaglandin 15-deoxy-Δ 12,14- PGJ 2 attenuates the development of colon injury caused by dinitrobenzene sulphonic acid in the rat. In: British Journal of Pharmacology. 2003 ; Vol. 138, No. 4. pp. 678-688.
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AU - Serraino, Ivana

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N2 - 1. Inflammatory bowel disease (IBD) is characterized by oxidative and nitrosative stress, leukocyte infiltration, and increased expression of the adhesion molecules intercellular adhesion molecule 1 (ICAM-1) in the colon. Recent evidence also suggests that the cyclopentenone prostaglandin (PG) 15-deoxy-Δ 12,14-PGJ 2 (15d- PGJ 2) functions as an early anti-inflammatory signal. 2. The aim of the present paper is to investigate the effects of 15d-PGJ 2 in rats subjected to experimental colitis. 3. Colitis was induced in rats by intra-colonic instillation of dinitrobenzene sulphonic acid (DNBS). 15d-PGJ 2 was administered daily as intraperitoneal injection (20 or 40 μg kg -1). On day 4, animals were sacrificed and tissues were taken for histological and biochemical analysis. 4. 15d-PGJ 2 significantly reduced the degree of haemorrhagic diarrhoea and weight loss caused by administration of DNBS. 15d-PGJ 2 also caused a substantial reduction of (i) the degree of colonic injury, (ii) the rise in myeloperoxidase (MPO) activity (mucosa), (iii) the increase in the tissue levels of malondialdehyde (MDA) and (iv) of the pro-inflammatory cytokines tumour necrosis factor-alpha (TNF-α) and interleukin-1β (IL-1β). 5. Furthermore, 15d-PGJ 2 reduced the increase in immunohistochemical staining for (i) inducible nitric oxide synthase (iNOS), (ii) nitrotyrosine and (iii) poly (ADP-ribose) polymerase (PARP), as well as (iv) the increased expression of ICAM-1 caused by DNBS in the colon. 6. Electrophoresis mobility shift assay (EMSA) of inflamed colon revealed that 15d- PGJ 2 also caused a substantial reduction of the activation of nuclear factor-kappaB (NF-κB). Furthermore, 15d-PGJ 2 stimulates the activation of heat shock protein 72 (hsp72) in the inflamed colon, as assessed by Western blot analysis. 7. In conclusion, 15d-PGJ 2 reduces the development of experimental colitis.

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