The daunorubicin-binding protein of Mr 54,000 is an aldehyde dehydrogenase and is down-regulated in mouse liver tumors and in tumor cell lines

Patrizia Banfi, Cinzia Lanzi, F. Stefania Falvella, Manuela Gariboldi, Romolo A. Gambetta, Tommaso A. Dragani

Research output: Contribution to journalArticle

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Abstract

A rabbit antiserum developed against purified rat liver daunorubicin-binding protein of Mr 54,000 (DNR-BP54) cross-reacted with a mouse protein of the same molecular weight. This protein was expressed in the liver and several other organs of mice. A series of tumors and cell lines tested for the presence of the protein were negative. By immunocytochemistry, we found that DNR-BP54 was abundantly expressed in the cytoplasm of normal hepatocytes but was expressed at much lower levels in urethane-induced mouse liver tumors. By immunoscreening of a mouse liver cDNA library, we cloned the cDNA coding for DNR-BP54 and we found that this protein is aldehyde dehydrogenase-2 (EC 1.2.1.3). This result was confirmed by the dehydrogenase activity found in pure preparations of DNR-BP54 from normal rat and mouse livers, assayed with acetaldehyde as substrate and NAD as cofactor. The enzyme activity was inhibited by daunorubicin. The inhibition was found to be competitive with respect to NAD.

Original languageEnglish
Pages (from-to)896-900
Number of pages5
JournalMolecular Pharmacology
Volume46
Issue number5
Publication statusPublished - Nov 1994

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Aldehyde Dehydrogenase
Daunorubicin
Tumor Cell Line
Carrier Proteins
Liver
Neoplasms
NAD
Proteins
Acetaldehyde
Urethane
Gene Library
Immune Sera
Hepatocytes
Oxidoreductases
Cytoplasm
Complementary DNA
Molecular Weight
Immunohistochemistry
Rabbits
Enzymes

ASJC Scopus subject areas

  • Pharmacology

Cite this

The daunorubicin-binding protein of Mr 54,000 is an aldehyde dehydrogenase and is down-regulated in mouse liver tumors and in tumor cell lines. / Banfi, Patrizia; Lanzi, Cinzia; Falvella, F. Stefania; Gariboldi, Manuela; Gambetta, Romolo A.; Dragani, Tommaso A.

In: Molecular Pharmacology, Vol. 46, No. 5, 11.1994, p. 896-900.

Research output: Contribution to journalArticle

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