The demonstration of acid α-naphthyl acetate esterase activity in human lymphocytes

Usefulness as a T-cell marker

D. M. Knowles, T. Hoffman, M. Ferrarini, H. G. Kunkel

Research output: Contribution to journalArticle

160 Citations (Scopus)

Abstract

The histochemical demonstration of nonspecific acid α-naphthyl acetate esterase (ANAE) activity was evaluated as a T lymphocyte marker primarily with the sheep erythrocyte (E) assay. A distinctive staining pattern characterized T lymphocytes which could be readily distinguished from monocyte staining. The percentage of E+ and ANAE+ lymphocytes was nearly always comparable in the peripheral blood and lymphoid tissue from normal and selected patients, including those with acute and chronic lymphocytic leukemia. Divergences were noted in certain other tissues including spleen and thymus. Certain mitogen-stimulated cells lost their ANAE activity while retaining their ability to form en rosettes. Atypical and variable staining patterns were observed in established lymphoid cell lines. The histochemical demonstration of ANAE is simple and reproducible; preparations may be counterstained for cytomorphologic detail and mounted as a permanent record. Certain disadvantages are discussed. The method represents a practical alternative to E rosette assays. It is particularly well suited for certain routine laboratories.

Original languageEnglish
Pages (from-to)112-123
Number of pages12
JournalCellular Immunology
Volume35
Issue number1
DOIs
Publication statusPublished - 1978

Fingerprint

Acetylesterase
Human Activities
Lymphocytes
T-Lymphocytes
Acids
Staining and Labeling
Lymphoid Tissue
B-Cell Chronic Lymphocytic Leukemia
Precursor Cell Lymphoblastic Leukemia-Lymphoma
Mitogens
Thymus Gland
Monocytes
Sheep
Spleen
Erythrocytes
Cell Line
naphthyl acetate

ASJC Scopus subject areas

  • Cell Biology
  • Immunology

Cite this

The demonstration of acid α-naphthyl acetate esterase activity in human lymphocytes : Usefulness as a T-cell marker. / Knowles, D. M.; Hoffman, T.; Ferrarini, M.; Kunkel, H. G.

In: Cellular Immunology, Vol. 35, No. 1, 1978, p. 112-123.

Research output: Contribution to journalArticle

Knowles, D. M. ; Hoffman, T. ; Ferrarini, M. ; Kunkel, H. G. / The demonstration of acid α-naphthyl acetate esterase activity in human lymphocytes : Usefulness as a T-cell marker. In: Cellular Immunology. 1978 ; Vol. 35, No. 1. pp. 112-123.
@article{6b5851e807b9416e9180478d60bb4453,
title = "The demonstration of acid α-naphthyl acetate esterase activity in human lymphocytes: Usefulness as a T-cell marker",
abstract = "The histochemical demonstration of nonspecific acid α-naphthyl acetate esterase (ANAE) activity was evaluated as a T lymphocyte marker primarily with the sheep erythrocyte (E) assay. A distinctive staining pattern characterized T lymphocytes which could be readily distinguished from monocyte staining. The percentage of E+ and ANAE+ lymphocytes was nearly always comparable in the peripheral blood and lymphoid tissue from normal and selected patients, including those with acute and chronic lymphocytic leukemia. Divergences were noted in certain other tissues including spleen and thymus. Certain mitogen-stimulated cells lost their ANAE activity while retaining their ability to form en rosettes. Atypical and variable staining patterns were observed in established lymphoid cell lines. The histochemical demonstration of ANAE is simple and reproducible; preparations may be counterstained for cytomorphologic detail and mounted as a permanent record. Certain disadvantages are discussed. The method represents a practical alternative to E rosette assays. It is particularly well suited for certain routine laboratories.",
author = "Knowles, {D. M.} and T. Hoffman and M. Ferrarini and Kunkel, {H. G.}",
year = "1978",
doi = "10.1016/0008-8749(78)90131-4",
language = "English",
volume = "35",
pages = "112--123",
journal = "Cellular Immunology",
issn = "0008-8749",
publisher = "Academic Press Inc.",
number = "1",

}

TY - JOUR

T1 - The demonstration of acid α-naphthyl acetate esterase activity in human lymphocytes

T2 - Usefulness as a T-cell marker

AU - Knowles, D. M.

AU - Hoffman, T.

AU - Ferrarini, M.

AU - Kunkel, H. G.

PY - 1978

Y1 - 1978

N2 - The histochemical demonstration of nonspecific acid α-naphthyl acetate esterase (ANAE) activity was evaluated as a T lymphocyte marker primarily with the sheep erythrocyte (E) assay. A distinctive staining pattern characterized T lymphocytes which could be readily distinguished from monocyte staining. The percentage of E+ and ANAE+ lymphocytes was nearly always comparable in the peripheral blood and lymphoid tissue from normal and selected patients, including those with acute and chronic lymphocytic leukemia. Divergences were noted in certain other tissues including spleen and thymus. Certain mitogen-stimulated cells lost their ANAE activity while retaining their ability to form en rosettes. Atypical and variable staining patterns were observed in established lymphoid cell lines. The histochemical demonstration of ANAE is simple and reproducible; preparations may be counterstained for cytomorphologic detail and mounted as a permanent record. Certain disadvantages are discussed. The method represents a practical alternative to E rosette assays. It is particularly well suited for certain routine laboratories.

AB - The histochemical demonstration of nonspecific acid α-naphthyl acetate esterase (ANAE) activity was evaluated as a T lymphocyte marker primarily with the sheep erythrocyte (E) assay. A distinctive staining pattern characterized T lymphocytes which could be readily distinguished from monocyte staining. The percentage of E+ and ANAE+ lymphocytes was nearly always comparable in the peripheral blood and lymphoid tissue from normal and selected patients, including those with acute and chronic lymphocytic leukemia. Divergences were noted in certain other tissues including spleen and thymus. Certain mitogen-stimulated cells lost their ANAE activity while retaining their ability to form en rosettes. Atypical and variable staining patterns were observed in established lymphoid cell lines. The histochemical demonstration of ANAE is simple and reproducible; preparations may be counterstained for cytomorphologic detail and mounted as a permanent record. Certain disadvantages are discussed. The method represents a practical alternative to E rosette assays. It is particularly well suited for certain routine laboratories.

UR - http://www.scopus.com/inward/record.url?scp=0017879418&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0017879418&partnerID=8YFLogxK

U2 - 10.1016/0008-8749(78)90131-4

DO - 10.1016/0008-8749(78)90131-4

M3 - Article

VL - 35

SP - 112

EP - 123

JO - Cellular Immunology

JF - Cellular Immunology

SN - 0008-8749

IS - 1

ER -