Abstract
Orcein stains granules in pancreatic islet cells selectively. The localization of Orcein-positive cells within islets differs from that of Grimelius-stained cells, but corresponds to the B cell type differentiated by Aldehyde Fuchsin. Usually there appear to be fewer Orcein-positive cells than Aldehyde Fuchsin-positive ones. This indicates either that Aldehyde Fuchsin is a more sensitive stain for B cells or that Orcein is a more selective stain for a B cell type subpopulation. The rationale of the Orcein reaction in B cells seems to depend on the oxidation of disulphide bonds present in insulin and its precursors rich in cystine.
Original language | English |
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Pages (from-to) | 545-552 |
Number of pages | 8 |
Journal | Histochemical Journal |
Volume | 14 |
Issue number | 4 |
DOIs | |
Publication status | Published - Jul 1982 |
ASJC Scopus subject areas
- Anatomy
- Cell Biology