The detection and biological activity of human antibodies to IL-2 in normal donors

L. Tiberio, A. Caruso, A. Pozzi, L. Rivoltini, D. Morelli, E. Monti, A. Balsari

Research output: Contribution to journalArticle

33 Citations (Scopus)

Abstract

Antibodies to interleukin-2 (IL-2) have been reported in the sera of patients under therapies involving this cytokine and in the sera of patients infected with human immunodeficiency virus (HIV). Our study proves that auto-antibodies to IL-2 are present also, at a lower titre, in healthy individuals. These antibodies were affinity purified and studied for their capability to interfere with the in vitro biological activity of IL-2. Data obtained show that human anti-IL-2 antibodies can interfere with lymphocyte proliferation both in the lymphokine activated killer (LAK) cell assay and in the mixed lymphocyte reaction (MLR). However, the kinetics of inhibition by anti-IL-2 antibodies differs from LAK cell assay to MLR as the former are always inhibited in a time-independent manner, and the latter only by adding antibodies at the outset of culture. The neutralizing activity observed in vitro suggests that such antibodies play a part in the elaborate cytokine network by which the immune system regulates the amplitude and duration of its response.

Original languageEnglish
Pages (from-to)472-476
Number of pages5
JournalScandinavian Journal of Immunology
Volume38
Issue number5
DOIs
Publication statusPublished - 1993

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Human Activities
Interleukin-2
Tissue Donors
Antibodies
Lymphokine-Activated Killer Cells
Mixed Lymphocyte Culture Test
Cytokines
Antibody Affinity
Serum
Immune System
HIV
Lymphocytes

ASJC Scopus subject areas

  • Immunology

Cite this

The detection and biological activity of human antibodies to IL-2 in normal donors. / Tiberio, L.; Caruso, A.; Pozzi, A.; Rivoltini, L.; Morelli, D.; Monti, E.; Balsari, A.

In: Scandinavian Journal of Immunology, Vol. 38, No. 5, 1993, p. 472-476.

Research output: Contribution to journalArticle

Tiberio, L. ; Caruso, A. ; Pozzi, A. ; Rivoltini, L. ; Morelli, D. ; Monti, E. ; Balsari, A. / The detection and biological activity of human antibodies to IL-2 in normal donors. In: Scandinavian Journal of Immunology. 1993 ; Vol. 38, No. 5. pp. 472-476.
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