The detection of intrathecal synthesis of anti-herpes simplex IgG antibodies: Comparison between an antigen-mediated immunoblotting technique and antibody index calculations

Philippe Monteyne, Frauke Albert, Benedikt Weissbrich, Elisabetta Zardini, Maria Ciardi, Graham M. Cleator, Christian J M Sindic

Research output: Contribution to journalArticlepeer-review

Abstract

The detection of intrathecal antibody synthesis was compared by the calculation of antibody indices (AI) derived from ELISA techniques with the detection of virus-specific oligoclonal IgGs by an antigen-mediated capillary blot technique. Twenty-seven paired serum and cerebrospinal fluid (CSF) samples were examined from 15 immunocompetent patients with herpes simplex virus encephalitis (HSE) diagnosed by PCR on early CSF samples. These techniques were also applied to paired samples from 20 multiple sclerosis (MS) patients, 10 patients with other inflammatory neurological diseases and 10 patients with non inflammatory neurological disorders. There was a good correlation between the results obtained by AI and those obtained by immunoblotting, especially in HSE (2 discordant results out of 27). Discrepancies were more frequent (25%) in MS patients where a 'polyspecific' reaction characterized by low affinity antibodies is known to occur. Some of the discrepancies could, in part, be due to serological cross-reaction with varicella zoster virus.

Original languageEnglish
Pages (from-to)324-331
Number of pages8
JournalJournal of Medical Virology
Volume53
Issue number4
DOIs
Publication statusPublished - Dec 1997

Keywords

  • Antibody indices
  • Cerebrospinal fluid
  • Herpes encephalitis
  • Intrathecal immunity
  • Oligoclonal antibodies

ASJC Scopus subject areas

  • Virology

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