The determination of ethanol n whoe blood by differential ph measurements

E. Rovida, M. Luzzana, M. Ripamonti, A. Mosca, V. Gambaro, E. Marozzi, F. Lodi, L. Rossi-Bernardi

Research output: Contribution to journalArticlepeer-review


The application of a new technique based on the differential measurement of pH between two solutions to determine ethanol concentration in whole blood is reported. The ethanol is determined by measuring the change in pH following its enzymatic oxidation to acetaldehyde. The procedure correlates with the head space gas chromatographic method in the 0-108 mmol/l (0-5 g/l) ethanol whole blood concentration range according to the equation y = 1344 + 1.013 × (r = 0.997).

Original languageEnglish
Pages (from-to)617-621
Number of pages5
JournalScandinavian Journal of Clinical and Laboratory Investigation
Issue number7
Publication statusPublished - 1984


  • Alcohol
  • Enzymatic assay
  • Head space gas chromatography

ASJC Scopus subject areas

  • Clinical Biochemistry


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