The development of axonal transport of proteins and glycoproteins in the optic pathway of chick embryos

Pier Carlo Marchisio, Johan Sjöstrand, Massimo Aglietta, Jan Olof Karlsson

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

Axonal transport in chick embryonic retinal neurons was studied after intraocular injection of [3H]proline or [3H]fucose. Rapid transport to the contralateral optic tectum could be demonstrated from the 10th day of incubation, and the rate of rapid transport increased with age. As compared to proline-labelled proteins, a relatively larger proportion of retinal fucose-labelled glycoproteins was transported to the tectum throughout the development, with the maximal difference at 18 days. Sodium dodecyl sulphate (SDS) gel electrophoresis of leucine- or fucose-labelled tectal proteins revealed that a large number of relatively high mol. wt. polypeptides reached the optic tectum by a rapid phase of axonal transport.

Original languageEnglish
Pages (from-to)273-284
Number of pages12
JournalBrain Research
Volume63
Issue numberC
DOIs
Publication statusPublished - Dec 7 1973

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Axonal Transport
Fucose
Chick Embryo
Glycoproteins
Carrier Proteins
Superior Colliculi
Proline
Intraocular Injections
Retinal Neurons
Leucine
Sodium Dodecyl Sulfate
Electrophoresis
Proteins
Gels
Peptides

ASJC Scopus subject areas

  • Developmental Biology
  • Molecular Biology
  • Clinical Neurology
  • Neuroscience(all)

Cite this

The development of axonal transport of proteins and glycoproteins in the optic pathway of chick embryos. / Marchisio, Pier Carlo; Sjöstrand, Johan; Aglietta, Massimo; Karlsson, Jan Olof.

In: Brain Research, Vol. 63, No. C, 07.12.1973, p. 273-284.

Research output: Contribution to journalArticle

Marchisio, Pier Carlo ; Sjöstrand, Johan ; Aglietta, Massimo ; Karlsson, Jan Olof. / The development of axonal transport of proteins and glycoproteins in the optic pathway of chick embryos. In: Brain Research. 1973 ; Vol. 63, No. C. pp. 273-284.
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