The development of neurogenic plasma extravasation in the rat dura mater does not depend upon the degranulation of mast cells

Stephen Markowitz, Kiyoshi Saito, Maria Gabriella Buzzi, Michael A. Moskowitz

Research output: Contribution to journalArticle

Abstract

Mast cells were visualized in stretch preparations of the rat dura mater and were found mostly in relation to small and large blood vessels. The overall number of dural mast cells was unaffected by electrical trigeminal or chemical deafferentation. As in other tissues, mast cell degranulation increased at sites of local injury (electrode penetration) or after systemic treatment with compound 48/80. However, mast cells did not degranulate following electrical trigeminal stimulation, or after injection of drugs (capsaicin or substance P) which promote plasma extravasation in the dura. Furthermore, pretreatment with a mast cell stabilizer (sodium dicromoglycate) or with large doses of H1 and H2 histamine receptor blockers (mepyramine and cimetidine), did not block electrically- or chemically-induced neurogenic plasma extravasation (NPE). Daily pretreatment with 48/80 however completely attenuated or abolished NPE. Taken together these data suggest that as assessed by the extrusion of metachromatic granules, mast cells are not essential to the development of neurogenic inflammation within the rat dura mater. However, these findings cannot exclude the possibility that mast cells may amplify or modulate this process.

Original languageEnglish
Pages (from-to)157-165
Number of pages9
JournalBrain Research
Volume477
Issue number1-2
DOIs
Publication statusPublished - Jan 16 1989

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Keywords

  • Compound 48/80
  • Dura
  • Mast cell
  • Neurogenic plasma extravasation
  • Trigeminal ganglion

ASJC Scopus subject areas

  • Developmental Biology
  • Molecular Biology
  • Clinical Neurology
  • Neuroscience(all)

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