The development of tissue-engineered bone of different origin through endochondral and intramembranous ossification following the implantation of mesenchymal stem cells and osteoblasts in a murine model

Federico Tortelli, Roberta Tasso, Fabrizio Loiacono, Ranieri Cancedda

Research output: Contribution to journalArticle

97 Citations (Scopus)

Abstract

The study of host cell recruitment by implanted exogenous cells is one of the novel challenges in tissue engineering. We previously reported the development of tissue-engineered bone deposited by host cells in porous ceramic scaffolds seeded with murine mesenchymal stem cells (MSC) and implanted in immunocompromised mice. To better highlight the contribution of host cells to the development of the engineered tissue and to investigate whether the capacity to recruit host cells was dependent on the donor cell commitment, we implanted ceramic scaffolds seeded with either murine GFP labeled MSC or GFP labeled osteoblasts (OB) into immunocompromised mice. Although we observed formation of bone in all scaffolds, the origin of bone cells and the ossification type were strictly dependent on the nature and commitment of the seeded cells. MSC implants led to formation of bone of host origin through the activation of an endochondral ossification process while an intramembranous ossification directly performed by the seeded cells was observed in OB implants. Moreover, we observed an increased vascularization in MSC implants due to the higher capacity of MSC to recruit host CD31+ endothelial cells. The relationship between this enhanced vascularization and the type of ossification is discussed.

Original languageEnglish
Pages (from-to)242-249
Number of pages8
JournalBiomaterials
Volume31
Issue number2
DOIs
Publication statusPublished - Jan 2010

Fingerprint

Osteoblasts
Stem cells
Mesenchymal Stromal Cells
Osteogenesis
Bone
Tissue
Bone and Bones
Scaffolds
Ceramics
Endothelial cells
Tissue engineering
Chemical activation
Tissue Engineering
Endothelial Cells

Keywords

  • Bone tissue engineering
  • Endothelial cell
  • Extracellular matrix (ECM)
  • Mesenchymal stem cell
  • Osteoblast
  • Osteogenesis

ASJC Scopus subject areas

  • Biophysics
  • Bioengineering
  • Mechanics of Materials
  • Biomaterials
  • Ceramics and Composites

Cite this

The development of tissue-engineered bone of different origin through endochondral and intramembranous ossification following the implantation of mesenchymal stem cells and osteoblasts in a murine model. / Tortelli, Federico; Tasso, Roberta; Loiacono, Fabrizio; Cancedda, Ranieri.

In: Biomaterials, Vol. 31, No. 2, 01.2010, p. 242-249.

Research output: Contribution to journalArticle

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