The disease associations of the antibody response against the Epstein-Barr virus transactivator protein ZEBRA can be separated into different epitopes

R. Tedeschi, Y. T. Foong, H. M. Cheng, P. DePaoli, T. Lehtinen, T. Elfborg, J. Dillner

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

The BamHI-Z-encoded Epstein-Barr virus (EBV) replication activator (ZEBRA) is a key mediator of the switch from latency to productive cycle in EBV virus. Antibodies against ZEBRA are a marker of EBV reactivation and are regularly found among patients with infectious mononucleosis (IM) or nasopharyngeal carcinoma (NPC), but are only rarely found among healthy EBV-seropositive donors. In order to define the serologically reactive epitopes in the ZEBRA protein, we synthesized a set of overlapping peptides and tested them for reactivity with serum samples from EBV-seronegative persons, patients with NPC, IM, chronic fatigue syndrome, lymphoma or from healthy donors. Three major EBV-specific epitopes were found. These epitopes were further defined and optimized using substitution or truncation analogues of the peptides. Reactivity with epitope number 22 was found in 63% of NPC patients' sera, with <2% of healthy donors' sera being positive. Serological reactivity with epitope number 19 was associated with IM (57% positive, 5% healthy donors positive). Serum antibodies against epitope 1 were found among healthy donors, but were significantly elevated among patients with NPC, IM or lymphomas. In conclusion, different serologically reactive epitopes in the ZEBRA protein associate with different EBV-associated diseases.

Original languageEnglish
Pages (from-to)1393-1400
Number of pages8
JournalJournal of General Virology
Volume76
Issue number6
Publication statusPublished - 1995

Fingerprint

Trans-Activators
Human Herpesvirus 4
Antibody Formation
Epitopes
Infectious Mononucleosis
Tissue Donors
Proteins
Serum
Lymphoma
Chronic Fatigue Syndrome
Peptides
Antibodies
Virus Diseases
Virus Replication
Viruses
Nasopharyngeal carcinoma

ASJC Scopus subject areas

  • Immunology
  • Virology

Cite this

The disease associations of the antibody response against the Epstein-Barr virus transactivator protein ZEBRA can be separated into different epitopes. / Tedeschi, R.; Foong, Y. T.; Cheng, H. M.; DePaoli, P.; Lehtinen, T.; Elfborg, T.; Dillner, J.

In: Journal of General Virology, Vol. 76, No. 6, 1995, p. 1393-1400.

Research output: Contribution to journalArticle

Tedeschi, R. ; Foong, Y. T. ; Cheng, H. M. ; DePaoli, P. ; Lehtinen, T. ; Elfborg, T. ; Dillner, J. / The disease associations of the antibody response against the Epstein-Barr virus transactivator protein ZEBRA can be separated into different epitopes. In: Journal of General Virology. 1995 ; Vol. 76, No. 6. pp. 1393-1400.
@article{9750af53cdbf49d0be6ae6f7f96616e8,
title = "The disease associations of the antibody response against the Epstein-Barr virus transactivator protein ZEBRA can be separated into different epitopes",
abstract = "The BamHI-Z-encoded Epstein-Barr virus (EBV) replication activator (ZEBRA) is a key mediator of the switch from latency to productive cycle in EBV virus. Antibodies against ZEBRA are a marker of EBV reactivation and are regularly found among patients with infectious mononucleosis (IM) or nasopharyngeal carcinoma (NPC), but are only rarely found among healthy EBV-seropositive donors. In order to define the serologically reactive epitopes in the ZEBRA protein, we synthesized a set of overlapping peptides and tested them for reactivity with serum samples from EBV-seronegative persons, patients with NPC, IM, chronic fatigue syndrome, lymphoma or from healthy donors. Three major EBV-specific epitopes were found. These epitopes were further defined and optimized using substitution or truncation analogues of the peptides. Reactivity with epitope number 22 was found in 63{\%} of NPC patients' sera, with <2{\%} of healthy donors' sera being positive. Serological reactivity with epitope number 19 was associated with IM (57{\%} positive, 5{\%} healthy donors positive). Serum antibodies against epitope 1 were found among healthy donors, but were significantly elevated among patients with NPC, IM or lymphomas. In conclusion, different serologically reactive epitopes in the ZEBRA protein associate with different EBV-associated diseases.",
author = "R. Tedeschi and Foong, {Y. T.} and Cheng, {H. M.} and P. DePaoli and T. Lehtinen and T. Elfborg and J. Dillner",
year = "1995",
language = "English",
volume = "76",
pages = "1393--1400",
journal = "Journal of General Virology",
issn = "0022-1317",
publisher = "Society for General Microbiology",
number = "6",

}

TY - JOUR

T1 - The disease associations of the antibody response against the Epstein-Barr virus transactivator protein ZEBRA can be separated into different epitopes

AU - Tedeschi, R.

AU - Foong, Y. T.

AU - Cheng, H. M.

AU - DePaoli, P.

AU - Lehtinen, T.

AU - Elfborg, T.

AU - Dillner, J.

PY - 1995

Y1 - 1995

N2 - The BamHI-Z-encoded Epstein-Barr virus (EBV) replication activator (ZEBRA) is a key mediator of the switch from latency to productive cycle in EBV virus. Antibodies against ZEBRA are a marker of EBV reactivation and are regularly found among patients with infectious mononucleosis (IM) or nasopharyngeal carcinoma (NPC), but are only rarely found among healthy EBV-seropositive donors. In order to define the serologically reactive epitopes in the ZEBRA protein, we synthesized a set of overlapping peptides and tested them for reactivity with serum samples from EBV-seronegative persons, patients with NPC, IM, chronic fatigue syndrome, lymphoma or from healthy donors. Three major EBV-specific epitopes were found. These epitopes were further defined and optimized using substitution or truncation analogues of the peptides. Reactivity with epitope number 22 was found in 63% of NPC patients' sera, with <2% of healthy donors' sera being positive. Serological reactivity with epitope number 19 was associated with IM (57% positive, 5% healthy donors positive). Serum antibodies against epitope 1 were found among healthy donors, but were significantly elevated among patients with NPC, IM or lymphomas. In conclusion, different serologically reactive epitopes in the ZEBRA protein associate with different EBV-associated diseases.

AB - The BamHI-Z-encoded Epstein-Barr virus (EBV) replication activator (ZEBRA) is a key mediator of the switch from latency to productive cycle in EBV virus. Antibodies against ZEBRA are a marker of EBV reactivation and are regularly found among patients with infectious mononucleosis (IM) or nasopharyngeal carcinoma (NPC), but are only rarely found among healthy EBV-seropositive donors. In order to define the serologically reactive epitopes in the ZEBRA protein, we synthesized a set of overlapping peptides and tested them for reactivity with serum samples from EBV-seronegative persons, patients with NPC, IM, chronic fatigue syndrome, lymphoma or from healthy donors. Three major EBV-specific epitopes were found. These epitopes were further defined and optimized using substitution or truncation analogues of the peptides. Reactivity with epitope number 22 was found in 63% of NPC patients' sera, with <2% of healthy donors' sera being positive. Serological reactivity with epitope number 19 was associated with IM (57% positive, 5% healthy donors positive). Serum antibodies against epitope 1 were found among healthy donors, but were significantly elevated among patients with NPC, IM or lymphomas. In conclusion, different serologically reactive epitopes in the ZEBRA protein associate with different EBV-associated diseases.

UR - http://www.scopus.com/inward/record.url?scp=0029021088&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0029021088&partnerID=8YFLogxK

M3 - Article

C2 - 7540196

AN - SCOPUS:0029021088

VL - 76

SP - 1393

EP - 1400

JO - Journal of General Virology

JF - Journal of General Virology

SN - 0022-1317

IS - 6

ER -