The DNA chaperone HMGB1 facilitates ACF/CHRAC-dependent nucleosome sliding

Tiziana Bonaldi, Gernot Längst, Ralf Strohner, Peter B. Becker, Marco E. Bianchi

Research output: Contribution to journalArticle

Abstract

Nucleosome remodelling complexes CHRAC and ACF contribute to chromatin dynamics by converting chemical energy into sliding of histone octamers on DNA. Their shared ATPase subunit ISWI binds DNA at the sites of entry into the nucleosome. A prevalent model assumes that DNA distortions catalysed by ISWI are converted into relocation of DNA relative to a histone octamer. HMGB1, one of the most abundant nuclear non-histone proteins, binds with preference to distorted DNA. We have now found that transient interaction of HMGB1 with nucleosomal linker DNA overlapping ISWI-binding sites enhances the ability of ACF to bind nucleosomal DNA and accelerates the sliding activity of limiting concentrations of remodelling factor. By contrast, an HMGB1 mutant with increased binding affinity was inhibitory. These observations are consistent with a role for HMGB1 as a DNA chaperone facilitating the rate-limiting DNA distortion during nucleosome remodelling.

Original languageEnglish
Pages (from-to)6865-6873
Number of pages9
JournalEMBO Journal
Volume21
Issue number24
DOIs
Publication statusPublished - Dec 16 2002

Keywords

  • ACF
  • Distortion
  • HMGB1
  • ISWI
  • Nucleosome sliding

ASJC Scopus subject areas

  • Genetics
  • Cell Biology

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