The DNA sequence of cosmid 14-13b from chromosome XIV of Saccharomyces cerevisiae reveals an unusually high number of overlapping open reading frames

Anna De Antoni, Michela D'Angelo, Francesca Dal Pero, Francesca Sartorello, Davide Pandolfo, Alberto Pallavicini, Gerolamo Lanfranchi, Giorgio Valle

Research output: Contribution to journalArticlepeer-review

Abstract

This work is part of the effort for sequencing chromosome XIV of Saccharomyces cerevisiae. Cosmid 14-13b contains a 37.8 kb insert derived from a partial Sau3A digestion of the genome, cloned into the BamHI site of the vector Pou6. The strategy used for sequencing is based on the fragmentation of the whole cosmid by sonication, followed by shotgun sequencing on an Applied Biosystem DNA sequencer. The clones with inserts corresponding to the vector were identified by dot-blot hybridization, without the need of sequencing. The analysis of the DNA sequence reveals 29 open reading frames (ORFs) longer than 300 bases. Nine ORFs are internal to some other ORFs. Similarity searches against DNA and protein data banks show that six ORFs correspond to already known yeast genes (OMP1, PSU1, MLS1, RPC19, DBP2, CYB5) and one ORF matches the sequence of a putative yeast gene (ESBP6). The cosmid sequence has been submitted to the EMBL data bank under Accession Number Z69382.

Original languageEnglish
Pages (from-to)261-266
Number of pages6
JournalYeast
Volume13
Issue number3
DOIs
Publication statusPublished - Mar 15 1997

Keywords

  • AF-9
  • chromosome XIV
  • CYB5
  • DBP2
  • ENL
  • ESBP6
  • genome sequencing
  • H8263
  • MLS1
  • OMP1
  • PSU1
  • RPC19
  • T BFI
  • TFIIF
  • yeast
  • YHR115c
  • YHR117w
  • YKL221w

ASJC Scopus subject areas

  • Agricultural and Biological Sciences (miscellaneous)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Biotechnology
  • Bioengineering
  • Microbiology

Fingerprint Dive into the research topics of 'The DNA sequence of cosmid 14-13b from chromosome XIV of Saccharomyces cerevisiae reveals an unusually high number of overlapping open reading frames'. Together they form a unique fingerprint.

Cite this