The ectopic FOF1 ATP synthase of rat liver is modulated in acute cholestasis by the inhibitor protein IF1

Valentina Giorgio, Elena Bisetto, Raffaella Franca, David A. Harris, Sabina Passamonti, Giovanna Lippe

Research output: Contribution to journalArticle

Abstract

Rat liver plasma membranes contain FOF1 complexes (ecto-FOF1) displaying a similar molecular weight to the mitochondrial FOF1 ATP synthase, as evidenced by Blue Native PAGE. Their ATPase activity was stably reduced in short-term extra-hepatic cholestasis. Immunoblotting and immunoprecipitation analyses demonstrated that the reduction in activity was not due to a decreased expression of ecto-FOF1 complexes, but to an increased level of an inhibitory protein, ecto-IF1, bound to ecto-F OF1. Since cholestasis down regulates the hepatic uptake of HDL-cholesterol, and ecto-FOF1 has been shown to mediate SR-BI-independent hepatic uptake of HDL-cholesterol, these findings provide support to the hypothesis that ecto-FOF1 contributes to the fine control of reverse cholesterol transport, in parallel with SR-BI. No activity change of the mitochondrial FOF1 ATP synthase (m-F OF1), or any variation of its association with m-IF 1 was observed in cholestasis, indicating that ecto-IF1 expression level is modulated independently from that of ecto-F OF1, m-IF1 and m-FOF1.

Original languageEnglish
Pages (from-to)117-123
Number of pages7
JournalJournal of Bioenergetics and Biomembranes
Volume42
Issue number2
DOIs
Publication statusPublished - Apr 2010

Fingerprint

Cholestasis
Adenosine Triphosphate
Mitochondrial Proton-Translocating ATPases
Liver
HDL Cholesterol
Proteins
Native Polyacrylamide Gel Electrophoresis
Immunoprecipitation
Immunoblotting
Adenosine Triphosphatases
Down-Regulation
Molecular Weight
Cholesterol
Cell Membrane

Keywords

  • Ectopic FF ATP synthase
  • Inhibitor protein IF
  • Rat liver
  • Short-term cholestasis

ASJC Scopus subject areas

  • Physiology
  • Cell Biology

Cite this

The ectopic FOF1 ATP synthase of rat liver is modulated in acute cholestasis by the inhibitor protein IF1 . / Giorgio, Valentina; Bisetto, Elena; Franca, Raffaella; Harris, David A.; Passamonti, Sabina; Lippe, Giovanna.

In: Journal of Bioenergetics and Biomembranes, Vol. 42, No. 2, 04.2010, p. 117-123.

Research output: Contribution to journalArticle

Giorgio, Valentina ; Bisetto, Elena ; Franca, Raffaella ; Harris, David A. ; Passamonti, Sabina ; Lippe, Giovanna. / The ectopic FOF1 ATP synthase of rat liver is modulated in acute cholestasis by the inhibitor protein IF1 . In: Journal of Bioenergetics and Biomembranes. 2010 ; Vol. 42, No. 2. pp. 117-123.
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