The effect of amyloid-β peptide on synaptic plasticity and memory is influenced by different isoforms, concentrations, and aggregation status

Walter Gulisano, Marcello Melone, Domenica D Li Puma, Maria Rosaria Tropea, Agostino Palmeri, Ottavio Arancio, Claudio Grassi, Fiorenzo Conti, Daniela Puzzo

Research output: Contribution to journalArticle

Abstract

The increase of oligomeric amyloid-beta (oAβ) has been related to synaptic dysfunction, thought to be the earliest event in Alzheimer's disease pathophysiology. Conversely, the suppression of endogenous Aβ impaired synaptic plasticity and memory, suggesting that the peptide is needed in the healthy brain. However, different species, aggregation forms and concentrations of Aβ might differently influence synaptic function/dysfunction. Here, we have tested the contribution of monomeric and oligomeric Aβ42 and Aβ40 at 200 nM and 200 pM concentrations on hippocampal long-term potentiation and spatial memory. We found that, when at 200 nM, oAβ40, oAβ42, and monomeric Aβ42 impaired long-term potentiation and memory, whereas only oAβ42 200 pM enhanced synaptic plasticity and memory and rescued the detrimental effect due to depletion of endogenous Aβ. Interestingly, quantification of monomer-like and oligomer-like species carried out by transmission electron microscopy revealed an increase of the monomer/oligomer ratio in the oAβ42 200 pM preparation, suggesting that the content of monomers and oligomers depends on the final concentration of the solution.

Original languageEnglish
Pages (from-to)51-60
Number of pages10
JournalNeurobiology of Aging
Volume71
DOIs
Publication statusPublished - Nov 2018

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Neuronal Plasticity
Long-Term Memory
Long-Term Potentiation
Amyloid
Protein Isoforms
Peptides
Transmission Electron Microscopy
Alzheimer Disease
Brain
Spatial Memory

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The effect of amyloid-β peptide on synaptic plasticity and memory is influenced by different isoforms, concentrations, and aggregation status. / Gulisano, Walter; Melone, Marcello; Li Puma, Domenica D; Tropea, Maria Rosaria; Palmeri, Agostino; Arancio, Ottavio; Grassi, Claudio; Conti, Fiorenzo; Puzzo, Daniela.

In: Neurobiology of Aging, Vol. 71, 11.2018, p. 51-60.

Research output: Contribution to journalArticle

Gulisano, Walter ; Melone, Marcello ; Li Puma, Domenica D ; Tropea, Maria Rosaria ; Palmeri, Agostino ; Arancio, Ottavio ; Grassi, Claudio ; Conti, Fiorenzo ; Puzzo, Daniela. / The effect of amyloid-β peptide on synaptic plasticity and memory is influenced by different isoforms, concentrations, and aggregation status. In: Neurobiology of Aging. 2018 ; Vol. 71. pp. 51-60.
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