The effect of clodronate on a mevalonate kinase deficiency cellular model

Valenti Zanin, Annalis Marcuzzi, Elisa Piscianz, Josef Vuch, Anna Monica Bianco, Lorenzo Monasta, Giuliana Decorti, Sergio Crovella

Research output: Contribution to journalArticle

Abstract

Background A potential anti-inflammatory effect of clodronate-an aminobisphosphonate-was described to antagonize the pro-inflammatory effects of the block in the mevalonate pathway, the main feature of a rare autoinflammatory disease called mevalonate kinase deficiency (MKD). Objective In this study we evaluated the potential anti-inflammatory effect of clodronate in MKD-a still orphan drug pediatric disease. Methods We studied some biological parameters, nitric oxide production using Griess reagents and programmed cell death by flow cytometry, as common inflammatory parameters in MKD, in the presence of different doses of clodronate (1, 10 and 100 lM). Results In our cellular model and in monocytes from patients with MKD, clodronate induced an increase in programed cell death and nitric oxide production in comparison with non-treated cells. Conclusion Our findings suggest that clodronate does not have an anti-inflammatory effect as previously reported but that it increases the epiphenomena of this pediatric disease.

Original languageEnglish
Pages (from-to)1363-1367
Number of pages5
JournalInflammation Research
Volume61
Issue number12
DOIs
Publication statusPublished - Dec 2012

Keywords

  • Apoptosis
  • Clodronate
  • Mevalonate kinase deficiency
  • Nitric oxide

ASJC Scopus subject areas

  • Pharmacology
  • Immunology

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