The effect of cytokines on human neutrophil Fc receptor-mediated phagocytosis

F. Capsoni, P. Bonara, F. Minonzio, A. M. Ongari, G. Colombo, G. P. Rizzardi, C. Zanussi

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Abstract

The recombinant (r) cytokines interferon-γ (rIFN-γ), granulocyte/macrophage-colony stimulating factor (rGM-CSF) and tumor necrosis factor-α (rTNF-α) all activate neutrophils. The aim of this work was to determine the effect of these cytokines on neutrophil Fc-receptor (FcR)-mediated phagocytosis and membrane expression of FcR, particularly FcRII and FcRIII. A short treatment (≥ 15 min) of neutrophils with rGM-CSF and rTNF-α at concentrations ≥ 62.5 U/ml significantly increased their ability to bind and phagocytize IgG-coated erythrocytes (EA). Both cytokines also showed more enhancing activity when suboptimally sensitized EA were used for binding and ingestion assays. A similar treatment of neutrophils with rIFN-γ at doses up to 500 U/ml was ineffective. The effect of rGM-CSF and rTNF-α was blocked by a monoclonal anti-GM-CSF antibody and by a polyclonal anti-TNF-α antibody respectively, thus establishing that the cytokines were responsible for the activity of the recombinant preparations. The cytokine-induced enhancement of FcR-mediated phagocytosis did not correlate with an enhancement of FcRII membrane expression on treated neutrophils; rGM-CSF significantly increased FcRIII expression, but rTNF-α and rIFN-γ were both ineffective in this respect. Since different roles of FcRII and FcRIII have been reported on ligand binding and ingestion, we also studied the effect of rGM-CSF and rTNF-α on the functional properties of these FcR, using specific monoclonal antibodies (mAbs). In the blocking experiments the pretreatment of neutrophils with rGM-CSF and rTNF-α did not modify the blocking properties of either anti-FcRII or anti-FcRIII mAbs, suggesting that cytokine-pretreatment does not affect the individual contribution of each type of FcR to ligand binding and internalization. Our data point to a new activity for both rGM-CSF and rTNF-α in augmenting FcR-mediated phagocytosis on neutrophils, but the mechanism of this enhancement remains to be elucidated.

Original languageEnglish
Pages (from-to)115-124
Number of pages10
JournalJournal of Clinical and Laboratory Immunology
Volume34
Issue number3
Publication statusPublished - 1991

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Fc Receptors
Granulocyte-Macrophage Colony-Stimulating Factor
Phagocytosis
Neutrophils
Cytokines
Tumor Necrosis Factor-alpha
Interferons
Eating
Monoclonal Antibodies
Ligands
Membranes
Anti-Idiotypic Antibodies
Immunoglobulin G
Erythrocytes
Antibodies

ASJC Scopus subject areas

  • Immunology

Cite this

Capsoni, F., Bonara, P., Minonzio, F., Ongari, A. M., Colombo, G., Rizzardi, G. P., & Zanussi, C. (1991). The effect of cytokines on human neutrophil Fc receptor-mediated phagocytosis. Journal of Clinical and Laboratory Immunology, 34(3), 115-124.

The effect of cytokines on human neutrophil Fc receptor-mediated phagocytosis. / Capsoni, F.; Bonara, P.; Minonzio, F.; Ongari, A. M.; Colombo, G.; Rizzardi, G. P.; Zanussi, C.

In: Journal of Clinical and Laboratory Immunology, Vol. 34, No. 3, 1991, p. 115-124.

Research output: Contribution to journalArticle

Capsoni, F, Bonara, P, Minonzio, F, Ongari, AM, Colombo, G, Rizzardi, GP & Zanussi, C 1991, 'The effect of cytokines on human neutrophil Fc receptor-mediated phagocytosis', Journal of Clinical and Laboratory Immunology, vol. 34, no. 3, pp. 115-124.
Capsoni, F. ; Bonara, P. ; Minonzio, F. ; Ongari, A. M. ; Colombo, G. ; Rizzardi, G. P. ; Zanussi, C. / The effect of cytokines on human neutrophil Fc receptor-mediated phagocytosis. In: Journal of Clinical and Laboratory Immunology. 1991 ; Vol. 34, No. 3. pp. 115-124.
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