The effect of Fgf-3/int-2 on growth and transformation of MCF-10A normal human mammary epithelial cells is distinct from FGF-1 and FGF-2

F. Basolo, T. Venesio, S. Calvo, L. Fiore, G. Fontanini, F. Ciardiello, A. Toniolo, D. S. Liscia, G. R. Merlo

Research output: Contribution to journalArticlepeer-review

Abstract

Fibroblast growth factors (FGFs) are related polypeptides with mitogenic activity on cells of mesodermal and neuroectodermal origin. The Fgf-3 gene shares high homology with FGF-2 and its protein product can substitute FGF-2 as a growth factor. Other observations, however, indicate that Fgf-3 has specialized functions. We have investigated the effect of the expression and secretion of Fgf-3 on the growth and transformation of the human breast epithelial cell line MCF-10A. Overexpression of Fgf-3 stimulates proliferation of these cells in serum-free medium and induces anchorage- independent colony formation in soft agar. In contrast, these effects were not observed with purified FGF-1 and FGF-2 on either the parental or the Fgf- 3-MCF-10A cells. Thus, Fgf-3 is distinct from FGF-1 and FGF-2 for its ability to induce cell proliferation and transformation of MCF-10A cells. This difference could be due, at least in part, to the expression of a specific set of FGF receptors with higher affinity for FGF-3 than FGF-1 or FGF-2.

Original languageEnglish
Pages (from-to)1365-1370
Number of pages6
JournalInternational Journal of Oncology
Volume4
Issue number6
Publication statusPublished - 1994

Keywords

  • Fgf-3
  • mammary cells
  • transformation

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

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